5vhh: Difference between revisions
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New page: '''Unreleased structure''' The entry 5vhh is ON HOLD Authors: Lu, Y., Wu, J., Dong, Y., Chen, S., Sun, S., Ma, Y.B., Ouyang, Q., Finley, D., Kirschner, M.W., Mao, Y. Description: Confo... |
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The | ==Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle== | ||
<SX load='5vhh' size='340' side='right' viewer='molstar' caption='[[5vhh]], [[Resolution|resolution]] 6.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5vhh]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VHH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VHH FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.1Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vhh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vhh OCA], [https://pdbe.org/5vhh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vhh RCSB], [https://www.ebi.ac.uk/pdbsum/5vhh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vhh ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PSMD3_HUMAN PSMD3_HUMAN] Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The proteasome holoenzyme is activated by its regulatory particle (RP) consisting of two subcomplexes, the lid and the base. A key event in base assembly is the formation of a heterohexameric ring of AAA-ATPases, which is guided by at least four RP assembly chaperones in mammals: PAAF1, p28/gankyrin, p27/PSMD9, and S5b. Using cryogenic electron microscopy, we analyzed the non-AAA structure of the p28-bound human RP at 4.5 A resolution and determined seven distinct conformations of the Rpn1-p28-AAA subcomplex within the p28-bound RP at subnanometer resolutions. Remarkably, the p28-bound AAA ring does not form a channel in the free RP and spontaneously samples multiple "open" and "closed" topologies at the Rpt2-Rpt6 and Rpt3-Rpt4 interfaces. Our analysis suggests that p28 assists the proteolytic core particle to select a specific conformation of the ATPase ring for RP engagement and is released in a shoehorn-like fashion in the last step of the chaperone-mediated proteasome assembly. | |||
Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle.,Lu Y, Wu J, Dong Y, Chen S, Sun S, Ma YB, Ouyang Q, Finley D, Kirschner MW, Mao Y Mol Cell. 2017 Jul 20;67(2):322-333.e6. doi: 10.1016/j.molcel.2017.06.007. Epub, 2017 Jul 6. PMID:28689658<ref>PMID:28689658</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5vhh" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Ankyrin 3D structures|Ankyrin 3D structures]] | ||
[[Category: | *[[Proteasome 3D structures|Proteasome 3D structures]] | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </SX> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | |||
[[Category: Chen S]] | |||
[[Category: Dong Y]] | |||
[[Category: Finley D]] | |||
[[Category: Kirschner MW]] | |||
[[Category: Lu Y]] | |||
[[Category: Ma YB]] | |||
[[Category: Mao Y]] | |||
[[Category: Ouyang Q]] | |||
[[Category: Sun S]] | |||
[[Category: Wu J]] | |||
Latest revision as of 10:07, 16 August 2023
Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle
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