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New page: ==Structure of human TUT7 catalytic module (CM)== <StructureSection load='5w0b' size='340' side='right' caption='5w0b, resolution 2.61Å' scene=''> == Structural h...
 
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==Structure of human TUT7 catalytic module (CM)==
==Structure of human TUT7 catalytic module (CM)==
<StructureSection load='5w0b' size='340' side='right' caption='[[5w0b]], [[Resolution|resolution]] 2.61&Aring;' scene=''>
<StructureSection load='5w0b' size='340' side='right'caption='[[5w0b]], [[Resolution|resolution]] 2.61&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5w0b]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W0B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W0B FirstGlance]. <br>
<table><tr><td colspan='2'>[[5w0b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W0B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W0B FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.614&#8491;</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_uridylyltransferase RNA uridylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.52 2.7.7.52] </span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w0b OCA], [http://pdbe.org/5w0b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w0b RCSB], [http://www.ebi.ac.uk/pdbsum/5w0b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w0b ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w0b OCA], [https://pdbe.org/5w0b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w0b RCSB], [https://www.ebi.ac.uk/pdbsum/5w0b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w0b ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/TUT7_HUMAN TUT7_HUMAN]] Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:19703396, PubMed:25480299). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (PubMed:25480299). Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A (PubMed:22898984). Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult.<ref>PMID:19703396</ref> <ref>PMID:22898984</ref> <ref>PMID:25480299</ref>
[https://www.uniprot.org/uniprot/TUT7_HUMAN TUT7_HUMAN] Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:19703396, PubMed:25480299). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (PubMed:25480299). Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A (PubMed:22898984). Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult.<ref>PMID:19703396</ref> <ref>PMID:22898984</ref> <ref>PMID:25480299</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The uridyl transferases TUT4 and TUT7 (collectively called TUT4(7)) switch between two modes of activity, either promoting expression of let-7 microRNA (monoU) or marking it for degradation (oligoU). Lin28 modulates the switch via recruitment of TUT4(7) to the precursor pre-let-7 in stem cells and human cancers. We found that TUT4(7) utilize two multidomain functional modules during the switch from monoU to oligoU. The catalytic module (CM) is essential for both activities, while the Lin28-interacting module (LIM) is indispensable for oligoU. A TUT7 CM structure trapped in the monoU activity staterevealed a duplex-RNA-binding pocket that orients group II pre-let-7 hairpins to favor monoU addition. Conversely, the switch to oligoU requires the ZK domain of Lin28 to drive the formation of a stable ternary complex between pre-let-7 and the inactive LIM. Finally, ZK2 of TUT4(7) aids oligoU addition by engaging the growing oligoU tail through uracil-specific interactions.


Multi-domain utilization by TUT4 and TUT7 in control of let-7 biogenesis.,Faehnle CR, Walleshauser J, Joshua-Tor L Nat Struct Mol Biol. 2017 Aug;24(8):658-665. doi: 10.1038/nsmb.3428. Epub 2017, Jul 3. PMID:28671666<ref>PMID:28671666</ref>
==See Also==
 
*[[RNA uridylyltransferase|RNA uridylyltransferase]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5w0b" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: RNA uridylyltransferase]]
[[Category: Homo sapiens]]
[[Category: Faehnle, C R]]
[[Category: Large Structures]]
[[Category: Joshua-Tor, L]]
[[Category: Faehnle CR]]
[[Category: Walleshauser, J]]
[[Category: Joshua-Tor L]]
[[Category: Terminal uridyltransferase]]
[[Category: Walleshauser J]]
[[Category: Transferase]]
[[Category: Tutase]]

Latest revision as of 14:13, 13 March 2024

Structure of human TUT7 catalytic module (CM)

5w0b, resolution 2.61Å

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