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[[Image:2fqf.jpg|left|200px]]


{{Structure
==Crystal Structures of E. coli Laccase CueO under different copper binding situations==
|PDB= 2fqf |SIZE=350|CAPTION= <scene name='initialview01'>2fqf</scene>, resolution 2.000&Aring;
<StructureSection load='2fqf' size='340' side='right'caption='[[2fqf]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>
<table><tr><td colspan='2'>[[2fqf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FQF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FQF FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fqf OCA], [https://pdbe.org/2fqf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fqf RCSB], [https://www.ebi.ac.uk/pdbsum/2fqf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fqf ProSAT]</span></td></tr>
|RELATEDENTRY=[[2fqd|2FQD]], [[2fqe|2FQE]], [[2fqg|2FQG]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fqf OCA], [http://www.ebi.ac.uk/pdbsum/2fqf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fqf RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/CUEO_ECOLI CUEO_ECOLI]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fq/2fqf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fqf ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CueO protein is a hypothetical bacterial laccase and a good laccase candidate for large scale industrial application. Four CueO crystal structures were determined at different copper concentrations. Low copper occupancy in apo-CueO and slow copper reconstitution process in CueO with exogenous copper were demonstrated. These observations well explain the copper dependence of CueO oxidase activity. Structural comparison between CueO and other three fungal laccase proteins indicates that Glu106 in CueO constitutes the primary counter-work for reconstitution of the trinuclear copper site. Mutation of Glu106 to a Phe enhanced CueO oxidation activity and supported this hypothesis. In addition, an extra alpha-helix from Leu351 to Gly378 covers substrate biding pocket of CueO and might compromises the electron transfer from substrate to type I copper.


'''Crystal Structures of E. coli Laccase CueO under different copper binding situations'''
Crystal structures of E. coli laccase CueO at different copper concentrations.,Li X, Wei Z, Zhang M, Peng X, Yu G, Teng M, Gong W Biochem Biophys Res Commun. 2007 Mar 2;354(1):21-6. Epub 2006 Dec 22. PMID:17217912<ref>PMID:17217912</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2fqf" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
CueO protein is a hypothetical bacterial laccase and a good laccase candidate for large scale industrial application. Four CueO crystal structures were determined at different copper concentrations. Low copper occupancy in apo-CueO and slow copper reconstitution process in CueO with exogenous copper were demonstrated. These observations well explain the copper dependence of CueO oxidase activity. Structural comparison between CueO and other three fungal laccase proteins indicates that Glu106 in CueO constitutes the primary counter-work for reconstitution of the trinuclear copper site. Mutation of Glu106 to a Phe enhanced CueO oxidation activity and supported this hypothesis. In addition, an extra alpha-helix from Leu351 to Gly378 covers substrate biding pocket of CueO and might compromises the electron transfer from substrate to type I copper.
*[[Blue copper oxidase CueO|Blue copper oxidase CueO]]
 
*[[Blue copper oxidase CueO 3D structures|Blue copper oxidase CueO 3D structures]]
==About this Structure==
== References ==
2FQF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FQF OCA].
<references/>
 
__TOC__
==Reference==
</StructureSection>
Crystal structures of E. coli laccase CueO at different copper concentrations., Li X, Wei Z, Zhang M, Peng X, Yu G, Teng M, Gong W, Biochem Biophys Res Commun. 2007 Mar 2;354(1):21-6. Epub 2006 Dec 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17217912 17217912]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Gong, W.]]
[[Category: Gong W]]
[[Category: Li, X.]]
[[Category: Li X]]
[[Category: Teng, M.]]
[[Category: Teng M]]
[[Category: Wei, Z.]]
[[Category: Wei Z]]
[[Category: Zhang, M.]]
[[Category: Zhang M]]
[[Category: azurin-like domain]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:06:22 2008''

Latest revision as of 08:03, 13 August 2026

Crystal Structures of E. coli Laccase CueO under different copper binding situations

2fqf, resolution 2.00Å

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