6cpv: Difference between revisions
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The | ==MicroED structure of NaK ion channel reveals a process of Na+ partition into the selectivity filter== | ||
<StructureSection load='6cpv' size='340' side='right'caption='[[6cpv]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6cpv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CPV FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 2.5Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cpv OCA], [https://pdbe.org/6cpv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cpv RCSB], [https://www.ebi.ac.uk/pdbsum/6cpv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cpv ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q81HW2_BACCR Q81HW2_BACCR] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Sodium (Na(+)) is a ubiquitous and important inorganic salt mediating many critical biological processes such as neuronal excitation, signaling, and facilitation of various transporters. The hydration states of Na(+) are proposed to play critical roles in determining the conductance and the selectivity of Na(+) channels, yet they are rarely captured by conventional structural biology means. Here we use the emerging cryo-electron microscopy (cryoEM) method micro-electron diffraction (MicroED) to study the structure of a prototypical tetrameric Na(+)-conducting channel, NaK, to 2.5 A resolution from nano-crystals. Two new conformations at the external site of NaK are identified, allowing us to visualize a partially hydrated Na(+) ion at the entrance of the channel pore. A process of dilation coupled with Na(+) movement is identified leading to valuable insights into the mechanism of ion conduction and gating. This study lays the ground work for future studies using MicroED in membrane protein biophysics. | |||
MicroED structure of the NaK ion channel reveals a Na(+) partition process into the selectivity filter.,Liu S, Gonen T Commun Biol. 2018;1:38. doi: 10.1038/s42003-018-0040-8. Epub 2018 May 3. PMID:30167468<ref>PMID:30167468</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6cpv" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Potassium channel 3D structures|Potassium channel 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bacillus cereus]] | |||
[[Category: Large Structures]] | |||
[[Category: Gonen T]] | |||
[[Category: Liu S]] | |||
Latest revision as of 15:08, 4 October 2023
MicroED structure of NaK ion channel reveals a process of Na+ partition into the selectivity filter
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