6g87: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: '''Unreleased structure''' The entry 6g87 is ON HOLD until Paper Publication Authors: Werner, N., Hoppen, J., Palm, G., Werten, S., Goettfert, M., Hinrichs, W. Description: Flavonoid-r... |
No edit summary |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Flavonoid-responsive Regulator FrrA== | |||
<StructureSection load='6g87' size='340' side='right'caption='[[6g87]], [[Resolution|resolution]] 2.92Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6g87]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/3i1b110 3i1b110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G87 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6G87 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CO678_15510 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1355477 3I1B110])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6g87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g87 OCA], [https://pdbe.org/6g87 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6g87 RCSB], [https://www.ebi.ac.uk/pdbsum/6g87 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6g87 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bradyrhizobium diazoefficiens, a bacterial symbiont of soybean and other leguminous plants, enters a nodulation-promoting genetic programme in the presence of host-produced flavonoids and related signalling compounds. Here, we describe the crystal structure of an isoflavonoid-responsive regulator (FrrA) from Bradyrhizobium, as well as cocrystal structures with inducing and noninducing ligands (genistein and naringenin, respectively). The structures reveal a TetR-like fold whose DNA-binding domain is capable of adopting a range of orientations. A single molecule of either genistein or naringenin is asymmetrically bound in a central cavity of the FrrA homodimer, mainly via C-H contacts to the pi-system of the ligands. Strikingly, however, the interaction does not provoke any conformational changes in the repressor. Both the flexible positioning of the DNA-binding domain and the absence of structural change upon ligand binding are corroborated by small-angle X-ray scattering (SAXS) experiments in solution. Together with a model of the promoter-bound state of FrrA our results suggest that inducers act as a wedge, preventing the DNA-binding domains from moving close enough together to interact with successive positions of the major groove of the palindromic operator. | |||
The induction mechanism of the flavonoid-responsive regulator FrrA.,Werner N, Werten S, Hoppen J, Palm GJ, Gottfert M, Hinrichs W FEBS J. 2021 Jul 27. doi: 10.1111/febs.16141. PMID:34314575<ref>PMID:34314575</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6g87" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Tetracycline repressor protein 3D structures|Tetracycline repressor protein 3D structures]] | |||
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Goettfert, M]] | |||
[[Category: Hinrichs, W]] | [[Category: Hinrichs, W]] | ||
[[Category: Hoppen, J]] | |||
[[Category: Palm, G]] | |||
[[Category: Werner, N]] | [[Category: Werner, N]] | ||
[[Category: Werten, S]] | [[Category: Werten, S]] | ||
[[Category: | [[Category: Flavonoid]] | ||
[[Category: Repressor]] | |||
[[Category: Tetr-family]] | |||
[[Category: Transcription]] | |||
Latest revision as of 06:13, 18 August 2021
Flavonoid-responsive Regulator FrrA
| ||||||||||||
