6dhx: Difference between revisions

(2 intermediate revisions by the same user not shown)

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 6dhx is ~~ON HOLD until Paper~~ Publication

==Structure of TipC2 from Streptococcus intermedius B196==

== Structural highlights ==

<table><tr><td colspan='2'>[[6dhx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_intermedius_b196 Streptococcus intermedius b196]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DHX FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SIR_1486 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=862967 Streptococcus intermedius B196])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dhx OCA], [http://pdbe.org/6dhx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dhx RCSB], [http://www.ebi.ac.uk/pdbsum/6dhx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dhx ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Gram-positive bacteria deploy the type VII secretion system (T7SS) to facilitate interactions between eukaryotic and prokaryotic cells. In recent work, we identified the TelC protein from Streptococcus intermedius as a T7SS-exported lipid II phosphatase that mediates interbacterial competition. TelC exerts toxicity in the inner wall zone of Gram-positive bacteria; however, intercellular intoxication of sister cells does not occur because they express the TipC immunity protein. In the present study, we sought to characterize the molecular basis of self-protection by TipC. Using sub-cellular localization and protease protection assays, we show that TipC is a membrane protein with an N-terminal transmembrane segment and a C-terminal TelC-inhibitory domain that protrudes into the inner wall zone. The 1.9A X-ray crystal structure of a non-protective TipC paralogue reveals that the soluble domain of TipC proteins adopts a crescent-shaped fold comprised of three alpha-helices and a seven-stranded beta-sheet. Subsequent homology-guided mutagenesis demonstrates that a concave surface formed by the predicted beta-sheet of TipC is required for both its interaction with TelC and its TelC-inhibitory activity. S. intermedius cells lacking the tipC gene are susceptible to growth inhibition by TelC delivered between cells; however, we find that the growth of this strain is unaffected by endogenous or overexpressed TelC even though the toxin accumulates in culture supernatants. Together, these data indicate that the TelC-inhibitory activity of TipC is only required for intercellularly-transferred TelC and that the T7SS apparatus transports TelC across the cell envelope in a single step, bypassing the cellular compartment in which it exerts toxicity en route.

~~Authors~~:

Molecular Basis for Immunity Protein Recognition of a Type VII Secretion System Exported Antibacterial Toxin.,Klein TA, Pazos M, Surette MG, Vollmer W, Whitney JC J Mol Biol. 2018 Sep 5. pii: S0022-2836(18)30594-1. doi:, 10.1016/j.jmb.2018.08.027. PMID:30194969<ref>PMID:30194969</ref>

~~Description~~:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased~~ Structures]]

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Streptococcus intermedius b196]]

[[Category: Bryant, D]]

[[Category: Klein, T A]]

[[Category: Whitney, J C]]

[[Category: Antitoxin]]

[[Category: Type vii secretion immunity protein]]

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6dhx is ON HOLD  until Paper Publication
+==Structure of TipC2 from Streptococcus intermedius B196==
+<StructureSection load='6dhx' size='340' side='right'caption='[[6dhx]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6dhx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_intermedius_b196 Streptococcus intermedius b196]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DHX FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SIR_1486 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=862967 Streptococcus intermedius B196])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dhx OCA], [http://pdbe.org/6dhx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dhx RCSB], [http://www.ebi.ac.uk/pdbsum/6dhx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dhx ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Gram-positive bacteria deploy the type VII secretion system (T7SS) to facilitate interactions between eukaryotic and prokaryotic cells. In recent work, we identified the TelC protein from Streptococcus intermedius as a T7SS-exported lipid II phosphatase that mediates interbacterial competition. TelC exerts toxicity in the inner wall zone of Gram-positive bacteria; however, intercellular intoxication of sister cells does not occur because they express the TipC immunity protein. In the present study, we sought to characterize the molecular basis of self-protection by TipC. Using sub-cellular localization and protease protection assays, we show that TipC is a membrane protein with an N-terminal transmembrane segment and a C-terminal TelC-inhibitory domain that protrudes into the inner wall zone. The 1.9A X-ray crystal structure of a non-protective TipC paralogue reveals that the soluble domain of TipC proteins adopts a crescent-shaped fold comprised of three alpha-helices and a seven-stranded beta-sheet. Subsequent homology-guided mutagenesis demonstrates that a concave surface formed by the predicted beta-sheet of TipC is required for both its interaction with TelC and its TelC-inhibitory activity. S. intermedius cells lacking the tipC gene are susceptible to growth inhibition by TelC delivered between cells; however, we find that the growth of this strain is unaffected by endogenous or overexpressed TelC even though the toxin accumulates in culture supernatants. Together, these data indicate that the TelC-inhibitory activity of TipC is only required for intercellularly-transferred TelC and that the T7SS apparatus transports TelC across the cell envelope in a single step, bypassing the cellular compartment in which it exerts toxicity en route.
-Authors:
+Molecular Basis for Immunity Protein Recognition of a Type VII Secretion System Exported Antibacterial Toxin.,Klein TA, Pazos M, Surette MG, Vollmer W, Whitney JC J Mol Biol. 2018 Sep 5. pii: S0022-2836(18)30594-1. doi:, 10.1016/j.jmb.2018.08.027. PMID:30194969<ref>PMID:30194969</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6dhx" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Streptococcus intermedius b196]]
+[[Category: Bryant, D]]
+[[Category: Klein, T A]]
+[[Category: Whitney, J C]]
+[[Category: Antitoxin]]
+[[Category: Type vii secretion immunity protein]]