6h62: Difference between revisions
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New page: '''Unreleased structure''' The entry 6h62 is ON HOLD Authors: Behrens, C., Heine, A., Reuter, K. Description: QTRT1, the catalytic subunit of murine tRNA-Guanine Transglycosylase [[Cat... |
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==QTRT1, the catalytic subunit of murine tRNA-Guanine Transglycosylase== | |||
<StructureSection load='6h62' size='340' side='right'caption='[[6h62]], [[Resolution|resolution]] 2.68Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6h62]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6H62 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.684Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6h62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h62 OCA], [https://pdbe.org/6h62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6h62 RCSB], [https://www.ebi.ac.uk/pdbsum/6h62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6h62 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/TGT_MOUSE TGT_MOUSE] Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:19414587, PubMed:29862811). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity).[HAMAP-Rule:MF_03218]<ref>PMID:19414587</ref> <ref>PMID:29862811</ref> | |||
==See Also== | |||
*[[TRNA-guanine transglycosylase 3D structures|TRNA-guanine transglycosylase 3D structures]] | |||
== References == | |||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: Heine | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Mus musculus]] | |||
[[Category: Behrens C]] | |||
[[Category: Heine A]] | |||
[[Category: Reuter K]] | |||
Latest revision as of 21:22, 28 June 2023
QTRT1, the catalytic subunit of murine tRNA-Guanine Transglycosylase
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