Malate synthase: Difference between revisions

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<StructureSection load='' size='350' side='right' caption='Structure of malate synthase G complex with CoA, malate, Hepes and Mg+2 ion (green) (PDB entry [[2gq3]])' scene='57/573146/Cv/1'>
<StructureSection load='' size='350' side='right' caption='Structure of malate synthase G complex with CoA, malate, Hepes and Mg+2 ion (green) (PDB entry [[2gq3]])' scene='57/573146/Cv/1'>
== Function ==     
== Function ==     
'''Malate synthase''' (MS) catalyzes the reversible conversion of acetyl-CoA, glyoxylate and water to (S)-malate and CoA.  MS participates in pyruvate, glyoxylate and dicarboxylate metabolism.  There are 2 isozymes of MS.  '''MS G''' is formed during growth on glycolate<ref>PMID:12930982</ref> and '''MS A''' which metabolizes glyoxylate formed in the dissimilation of acetate.
'''Malate synthase''' (MS) catalyzes the reversible conversion of acetyl-CoA, glyoxylate and water to [[R/S nomenclature|(S)]]-malate and CoA.  MS participates in pyruvate, glyoxylate and dicarboxylate metabolism.  There are 2 isozymes of MS.  '''MS G''' is formed during growth on glycolate<ref>PMID:12930982</ref> and '''MS A''' which metabolizes glyoxylate formed in the dissimilation of acetate.
 
See also: [[Glyoxylate cycle]]


== Structural highlights ==
== Structural highlights ==
<scene name='57/573146/Cv/7'>MS active site pocket is situated between the TIM barrel and the C-terminal</scene>.  The ternary complex contains malate, acetyl-CoA and Mg+2 ion<ref>PMID:16877713</ref>.  
<scene name='57/573146/Cv/8'>MS active site pocket is situated between the TIM barrel and the C-terminal</scene>.  The ternary complex contains malate, acetyl-CoA and Mg+2 ion<ref>PMID:16877713</ref>.  


<scene name='57/573146/Cv/3'>Malate/Mg+2 ion binding site</scene>. Water molecules shown as red spheres.
<scene name='57/573146/Cv/9'>Malate/Mg+2 ion binding site</scene>. Water molecules are shown as red spheres.


<scene name='57/573146/Cv/6'>Acetyl-CoA binding site</scene>.
<scene name='57/573146/Cv/10'>Acetyl-CoA binding site</scene>.
</StructureSection>


==3d structures of malate synthase==
==3d structures of malate synthase==
[[Malate synthase 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
</StructureSection>
{{#tree:id=OrganizedByTopic|openlevels=0|
 
*'''Malate synthase'''
 
**[[1y8b]], [[2jqx]] – EcMS G (mutant) – ''Escherichia coli'' - NMR<br />
**[[3cuz]], [[3cv1]] – EcMS A<br />
**[[4ex4]] – MS G + Mg – ''Mycobacterium leprae''<br />
**[[5h8p]] – MtMS G  – ''Mycobacterium tuberculosis''<br />
**[[5h8m]] – MtMS G (mutant) + Mg <br />
**[[3cux]] – MS + Mg – ''Bacillus anthracis''<br />
**[[5oas]] – PaMS G + Mg – ''Pseudomonas aeruginosa''<br />
 
*Malate synthase binary complex
 
**[[1d8c]] – EcMS G (mutant) + glyoxylate + Mg <br />
**[[3cv2]] – EcMS A + oxalate + Mg <br />
**[[1n8i]] – MtMS G + glyoxylate + Mg <br />
**[[5h8u]] – MtMS G + malate + Mg <br />
**[[56axe]] – MtMS G + acetyl CoA + Mg <br />
**[[5c7v]], [[5cah]], [[5cjm]], [[5e9x]] – MtMS G (mutant) + pyrrole derivative + Mg <br />
**[[5cew]] – MtMS G (mutant) + pyridine derivative + Mg <br />
**[[5cjn]] – MtMS G (mutant) + quinoxaline derivative + Mg <br />
**[[5dx7]] – MtMS G (mutant) + isoxazole derivative + Mg <br />
**[[5c9r]], [[5c9u]], [[5c9w]], [[5cbj]], [[5ccz]] – MtMS G (mutant) + phenyl derivative + Mg <br />
**[[5c9x]], [[5cbi]] – MtMS G (mutant) + benzoate derivative + Mg <br />
**[[5cak]], [[5cbb]], [[5cc3]], [[5cc5]], [[5cc6]], [[5cc7]], [[5drc]], [[5dri]], [[5ecv]] – MtMS G (mutant) + indole derivative + Mg <br />
**[[5t8g]] – MtMS G (mutant) + thiophene derivative + Mg <br />
**[[3s9i]], [[3s9z]], [[3sad]], [[3saz]], [[3sb0]] – MtMS G (mutant) + inhibitor + Mg <br />
**[[3oyx]], [[3pug]] – HvMS G + glyoxylate + Mg – ''Haloferax volcanii''<br />
**[[5tao]] – HvMS G + glyoxylate + Pb<br />
**[[5vfb]] – PaMS + glycolate + Ni<br />
 
*Malate synthase ternary complex


**[[1p7t]] – EcMS G (mutant) + pyruvate + Mg + acetyl-CoA<br />
**[[3oyz]] – HvMS + pyruvate + Mg + acetyl-CoA<br />
**[[1n8w]] – MtMS G + glyoxylate + Mg + CoA<br />
**[[2gq3]] – MtMS G + malate + Mg + CoA<br />
}}
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 09:03, 11 January 2023

Structure of malate synthase G complex with CoA, malate, Hepes and Mg+2 ion (green) (PDB entry 2gq3)

Drag the structure with the mouse to rotate

References

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Karsten Theis, Joel L. Sussman

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