6j2n: Difference between revisions
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New page: '''Unreleased structure''' The entry 6j2n is ON HOLD Authors: Yao, C. Description: Substrate-processing C3-b Category: Unreleased Structures Category: Yao, C |
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The | ==yeast proteasome in substrate-processing state (C3-b)== | ||
<SX load='6j2n' size='340' side='right' viewer='molstar' caption='[[6j2n]], [[Resolution|resolution]] 7.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6j2n]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J2N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J2N FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.5Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j2n OCA], [https://pdbe.org/6j2n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j2n RCSB], [https://www.ebi.ac.uk/pdbsum/6j2n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j2n ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RPN9_YEAST RPN9_YEAST] Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The 26S proteasome is the ATP-dependent protease responsible for regulating the proteome of eukaryotic cells through degradation of mainly ubiquitin-tagged substrates. In order to understand how proteasome responds to ubiquitin signal, we resolved an ensemble of cryo-EM structures of proteasome in the presence of K48-Ub4, with three of them resolved at near-atomic resolution. We identified a conformation with stabilized ubiquitin receptors and a previously unreported orientation of the lid, assigned as a Ub-accepted state C1-b. We determined another structure C3-b with localized K48-Ub4 to the toroid region of Rpn1, assigned as a substrate-processing state. Our structures indicate that tetraUb induced conformational changes in proteasome could initiate substrate degradation. We also propose a CP gate-opening mechanism involving the propagation of the motion of the lid to the gate through the Rpn6-alpha2 interaction. Our results enabled us to put forward a model of a functional cycle for proteasomes induced by tetraUb and nucleotide. | |||
Structural Snapshots of 26S Proteasome Reveal Tetraubiquitin-Induced Conformations.,Ding Z, Xu C, Sahu I, Wang Y, Fu Z, Huang M, Wong CCL, Glickman MH, Cong Y Mol Cell. 2019 Feb 12. pii: S1097-2765(19)30038-3. doi:, 10.1016/j.molcel.2019.01.018. PMID:30792173<ref>PMID:30792173</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6j2n" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Proteasome 3D structures|Proteasome 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Large Structures]] | |||
[[Category: Saccharomyces cerevisiae S288C]] | |||
[[Category: Cong Y]] | |||
Latest revision as of 10:05, 23 October 2024
yeast proteasome in substrate-processing state (C3-b)
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