6nmj: Difference between revisions
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==Crystal Structure of Rat Ric-8A G alpha binding domain, "Paratone-N Immersed"== | |||
<StructureSection load='6nmj' size='340' side='right'caption='[[6nmj]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6nmj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NMJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NMJ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nmj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nmj OCA], [https://pdbe.org/6nmj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nmj RCSB], [https://www.ebi.ac.uk/pdbsum/6nmj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nmj ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B1H241_RAT B1H241_RAT] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Ric-8A is a 530-amino acid cytoplasmic molecular chaperone and guanine nucleotide exchange factor (GEF) for i, q, and 12/13 classes of heterortrimeric G protein alpha subunits (Galpha). We report the 2.2-A crystal structure of the Ric-8A Galpha-binding domain with GEF activity, residues 1-452, and is phosphorylated at Ser435 and Thr440. Residues 1-429 adopt a superhelical fold comprised of Armadillo (ARM) and HEAT repeats, and the C terminus is disordered. One of the phosphorylated residues potentially binds to a basic cluster in an ARM motif. Amino acid sequence conservation and published hydrogen-deuterium exchange data indicate repeats 3 through 6 to be a putative Galpha-binding surface. Normal mode modeling of small-angle X-ray scattering data indicates that phosphorylation induces relative rotation between repeats 1-4, 5-6, and 7-9. 2D (1)H-(15)N-TROSY spectra of [(2)H,(15)N]-labeled Galphai1 in the presence of R452 reveals chemical shift perturbations of the C terminus and Galphai1 residues involved in nucleotide binding. | |||
Structure, Function, and Dynamics of the Galpha Binding Domain of Ric-8A.,Zeng B, Mou TC, Doukov TI, Steiner A, Yu W, Papasergi-Scott M, Tall GG, Hagn F, Sprang SR Structure. 2019 Jul 2;27(7):1137-1147.e5. doi: 10.1016/j.str.2019.04.013. Epub, 2019 May 30. PMID:31155309<ref>PMID:31155309</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6nmj" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Rattus norvegicus]] | |||
[[Category: Mou TC]] | |||
[[Category: Sprang SR]] | |||
[[Category: Zeng B]] | |||
Latest revision as of 06:56, 11 October 2023
Crystal Structure of Rat Ric-8A G alpha binding domain, "Paratone-N Immersed"
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