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[[Image:1dj0.jpg|left|200px]]


<!--
==THE CRYSTAL STRUCTURE OF E. COLI PSEUDOURIDINE SYNTHASE I AT 1.5 ANGSTROM RESOLUTION==
The line below this paragraph, containing "STRUCTURE_1dj0", creates the "Structure Box" on the page.
<StructureSection load='1dj0' size='340' side='right'caption='[[1dj0]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1dj0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DJ0 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
{{STRUCTURE_1dj0|  PDB=1dj0 |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dj0 OCA], [https://pdbe.org/1dj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dj0 RCSB], [https://www.ebi.ac.uk/pdbsum/1dj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dj0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TRUA_ECOLI TRUA_ECOLI] Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.<ref>PMID:17466622</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dj/1dj0_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dj0 ConSurf].
<div style="clear:both"></div>


'''THE CRYSTAL STRUCTURE OF E. COLI PSEUDOURIDINE SYNTHASE I AT 1.5 ANGSTROM RESOLUTION'''
==See Also==
 
*[[Guide-independent Pseudouridine synthase|Guide-independent Pseudouridine synthase]]
 
*[[Pseudouridine synthase 3D structures|Pseudouridine synthase 3D structures]]
==Overview==
== References ==
Pseudouridine synthases catalyze the isomerization of specific uridines to pseudouridine in a variety of RNAs, yet the basis for recognition of the RNA sites or how they catalyze this reaction is unknown. The crystal structure of pseudouridine synthase I from Escherichia coli, which, for example, modifies positions 38, 39 and/or 40 in tRNA, reveals a dimeric protein that contains two positively charged, RNA-binding clefts along the surface of the protein. Each cleft contains a highly conserved aspartic acid located at its center. The structural domains have a topological similarity to those of other RNA-binding proteins, though the mode of interaction with tRNA appears to be unique. The structure suggests that a dimeric enzyme is required for binding transfer RNA and subsequent pseudouridine formation.
<references/>
 
__TOC__
==About this Structure==
</StructureSection>
1DJ0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJ0 OCA].
 
==Reference==
The structural basis for tRNA recognition and pseudouridine formation by pseudouridine synthase I., Foster PG, Huang L, Santi DV, Stroud RM, Nat Struct Biol. 2000 Jan;7(1):23-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10625422 10625422]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Pseudouridylate synthase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Foster PG]]
[[Category: Foster, P G.]]
[[Category: Huang L]]
[[Category: Huang, L.]]
[[Category: Santi DV]]
[[Category: Santi, D V.]]
[[Category: Stroud RM]]
[[Category: Stroud, R M.]]
[[Category: Alpha/beta fold]]
[[Category: Rna-binding motif]]
[[Category: Rna-modifying enzyme]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:54:03 2008''

Latest revision as of 06:54, 7 February 2024

THE CRYSTAL STRUCTURE OF E. COLI PSEUDOURIDINE SYNTHASE I AT 1.5 ANGSTROM RESOLUTION

1dj0, resolution 1.50Å

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