7b5i: Difference between revisions
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==Cryo-EM structure of the contractile injection system cap complex from Anabaena PCC7120== | |||
<StructureSection load='7b5i' size='340' side='right'caption='[[7b5i]], [[Resolution|resolution]] 2.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7b5i]] is a 30 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B5I FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b5i OCA], [https://pdbe.org/7b5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b5i RCSB], [https://www.ebi.ac.uk/pdbsum/7b5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b5i ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8YRW5_NOSS1 Q8YRW5_NOSS1] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Contractile injection systems (CISs) mediate cell-cell interactions by phage tail-like structures, using two distinct modes of action: extracellular CISs are released into the medium, while type 6 secretion systems (T6SSs) are attached to the cytoplasmic membrane and function upon cell-cell contact. Here, we characterized a CIS in the multicellular cyanobacterium Anabaena, with features distinct from extracellular CISs and T6SSs. Cryo-electron tomography of focused ion beam-milled cells revealed that CISs were anchored in thylakoid membrane stacks, facing the cell periphery. Single particle cryo-electron microscopy showed that this unique in situ localization was mediated by extensions of tail fibre and baseplate components. On stress, cyanobacteria induced the formation of ghost cells, presenting thylakoid-anchored CISs to the environment. Functional assays suggest that these CISs may mediate ghost cell formation and/or interactions of ghost cells with other organisms. Collectively, these data provide a framework for understanding the evolutionary re-engineering of CISs and potential roles of these CISs in cyanobacterial programmed cell death. | |||
Structure of a thylakoid-anchored contractile injection system in multicellular cyanobacteria.,Weiss GL, Eisenstein F, Kieninger AK, Xu J, Minas HA, Gerber M, Feldmuller M, Maldener I, Forchhammer K, Pilhofer M Nat Microbiol. 2022 Mar;7(3):386-396. doi: 10.1038/s41564-021-01055-y. Epub 2022 , Feb 14. PMID:35165386<ref>PMID:35165386</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7b5i" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Nostoc sp. PCC 7120 = FACHB-418]] | |||
[[Category: Eisenstein F]] | |||
[[Category: Pilhofer M]] | |||
[[Category: Weiss GL]] | |||
Latest revision as of 06:05, 21 November 2024
Cryo-EM structure of the contractile injection system cap complex from Anabaena PCC7120
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