1evo: Difference between revisions
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==NMR OBSERVATION OF A NOVEL C-TETRAD== | |||
<StructureSection load='1evo' size='340' side='right'caption='[[1evo]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1evo]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EVO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EVO FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1evo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1evo OCA], [https://pdbe.org/1evo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1evo RCSB], [https://www.ebi.ac.uk/pdbsum/1evo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1evo ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
''' | == Publication Abstract from PubMed == | ||
== | |||
We report the NMR structure of the DNA sequence d-TGGGCGGT in Na(+) solutions at neutral pH, containing a repeat sequence from SV40 viral genome. The structure is a novel quadruplex incorporating the C-tetrad formed by symmetrical pairing of four Cs via NH(2)&bond;O(2) H-bonds in a plane. The C-tetrad has a wider cavity compared to G-tetrads and stacks well over the adjacent G4-tetrad, but poorly on the G6 tetrad. The quadruplex helix is largely underwound by 8-10 degrees compared to B-DNA except at the C5-G6 step. To our knowledge this is the first report of C-tetrad formation in DNA structures, and would be of significance from the point of view of both structural diversity and specific recognition. | We report the NMR structure of the DNA sequence d-TGGGCGGT in Na(+) solutions at neutral pH, containing a repeat sequence from SV40 viral genome. The structure is a novel quadruplex incorporating the C-tetrad formed by symmetrical pairing of four Cs via NH(2)&bond;O(2) H-bonds in a plane. The C-tetrad has a wider cavity compared to G-tetrads and stacks well over the adjacent G4-tetrad, but poorly on the G6 tetrad. The quadruplex helix is largely underwound by 8-10 degrees compared to B-DNA except at the C5-G6 step. To our knowledge this is the first report of C-tetrad formation in DNA structures, and would be of significance from the point of view of both structural diversity and specific recognition. | ||
NMR observation of a novel C-tetrad in the structure of the SV40 repeat sequence GGGCGG.,Patel PK, Bhavesh NS, Hosur RV Biochem Biophys Res Commun. 2000 Apr 21;270(3):967-71. PMID:10772934<ref>PMID:10772934</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1evo" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Bhavesh NS]] | ||
[[Category: | [[Category: Hosur RV]] | ||
[[Category: Patel PK]] | |||
Latest revision as of 11:48, 22 November 2023
NMR OBSERVATION OF A NOVEL C-TETRAD
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