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New page: left|200px<br /> <applet load="1ehw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ehw, resolution 2.40Å" /> '''HUMAN NUCLEOSIDE DI...
 
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[[Image:1ehw.gif|left|200px]]<br />
<applet load="1ehw" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ehw, resolution 2.40&Aring;" />
'''HUMAN NUCLEOSIDE DIPHOSPHATE KINASE 4'''<br />


==Overview==
==HUMAN NUCLEOSIDE DIPHOSPHATE KINASE 4==
We demonstrate here the catalytic activity and subcellular localization of, the Nm23-H4 protein, product of nm23-H4, a new member of the human, nm23/nucleoside diphosphate (NDP) kinase gene family (Milon, L., Rousseau-Merck, M., Munier, A., Erent, M., Lascu, I., Capeau, J., and, Lacombe, M. L. (1997) Hum. Genet. 99, 550-557). Nm3-H4 was synthesized in, escherichia coli as the full-length protein and as a truncated form, missing the N-terminal extension characteristic of mitochondrial, targeting. The truncated form possesses NDP kinase activity, whereas the, full-length protein is inactive, suggesting that the extension prevents, enzyme folding and/or activity. X-ray crystallographic analysis was, performed on active truncated Nm23-H4. Like other eukaryotic NDP kinases, it is a hexamer. Nm23-H4 naturally possesses a serine residue at position, 129, equivalent to the K-pn mutation of the Drosophila NDP kinase. The, x-ray structure shows that the presence of Ser(129) has local structural, effects that weaken subunit interactions. Site-directed mutagenesis shows, that the serine is responsible for the lability of Nm23-H4 to heat and, urea treatment, because the S129P mutant is greatly stabilized., Examination of human embryonic kidney 293 cells transfected with green, fluorescent protein fusions by confocal microscopy shows a specific, mitochondrial localization of Nm23-H4 that was also demonstrated by, Western blot analysis of subcellular fractions of these cells. Import into, mitochondria is accompanied by cleavage of the N-terminal extension that, results in NDP kinase activity. Submitochondrial fractionation indicates, that Nm23-H4 is associated with mitochondrial membranes, possibly to the, contact sites between the outer and inner membranes.
<StructureSection load='1ehw' size='340' side='right'caption='[[1ehw]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ehw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EHW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ehw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ehw OCA], [https://pdbe.org/1ehw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ehw RCSB], [https://www.ebi.ac.uk/pdbsum/1ehw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ehw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NDKM_HUMAN NDKM_HUMAN] Major role in the synthesis of nucleoside triphosphates other than ATP (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eh/1ehw_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ehw ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1EHW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EHW OCA].
*[[Nucleoside diphosphate kinase 3D structures|Nucleoside diphosphate kinase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The human nm23-H4 gene product is a mitochondrial nucleoside diphosphate kinase., Milon L, Meyer P, Chiadmi M, Munier A, Johansson M, Karlsson A, Lascu I, Capeau J, Janin J, Lacombe ML, J Biol Chem. 2000 May 12;275(19):14264-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10799505 10799505]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Nucleoside-diphosphate kinase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Capeau J]]
[[Category: Capeau, J.]]
[[Category: Chiadmi M]]
[[Category: Chiadmi, M.]]
[[Category: Janin J]]
[[Category: Janin, J.]]
[[Category: Johansson M]]
[[Category: Johansson, M.]]
[[Category: Karlsson A]]
[[Category: Karlsson, A.]]
[[Category: Lacombe M-L]]
[[Category: Lacombe, M-L.]]
[[Category: Lascu I]]
[[Category: Lascu, I.]]
[[Category: Meyer P]]
[[Category: Meyer, P.]]
[[Category: Milon L]]
[[Category: Milon, L.]]
[[Category: Munier A]]
[[Category: Munier, A.]]
[[Category: SO4]]
[[Category: killer-of-prune]]
[[Category: mitochondrial]]
[[Category: nm23]]
[[Category: nucleoside diphosphate kinase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:43:44 2007''

Latest revision as of 07:03, 7 February 2024

HUMAN NUCLEOSIDE DIPHOSPHATE KINASE 4

1ehw, resolution 2.40Å

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