3owg

Crystal structure of vaccinia virus Polyadenylate polymerase(vp55)

spinqualitylabelsall models 3owg, resolution 2.86Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3owg is a 2 chain structure with sequence from Vaccinia virus WR. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.86Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PAP1_VACCW Polymerase that creates the 3'-poly(A) tail of mRNA's. Publication Abstract from PubMed Vaccinia virus poly(A) polymerase (VP55) is the only known polymerase that can translocate independently with respect to single-stranded nucleic acid (ssNA). Previously, its structure has only been solved in the context of the VP39 processivity factor. Here, a crystal structure of unliganded monomeric VP55 has been solved to 2.86 A resolution, showing the first backbone structural isoforms among either VP55 or its processivity factor (VP39). Backbone differences between the two molecules of VP55 in the asymmetric unit indicated that unliganded monomeric VP55 can undergo a `rocking' motion of the N-terminal domain with respect to the other two domains, which may be `rigidified' upon VP39 docking. This observation is consistent with previously demonstrated experimental molecular dynamics of the monomer during translocation with respect to nucleic acid and with different mechanisms of translocation in the presence and absence of processivity factor VP39. Side-chain conformational changes in the absence of ligand were observed at a key primer contact site and at the catalytic center of VP55. The current structure completes the trio of possible structural forms for VP55 and VP39, namely the VP39 monomer, the VP39-VP55 heterodimer and the VP55 monomer. Domain-level rocking motion within a polymerase that translocates on single-stranded nucleic acid.,Li H, Li C, Zhou S, Poulos TL, Gershon PD Acta Crystallogr D Biol Crystallogr. 2013 Apr;69(Pt 4):617-24. doi:, 10.1107/S0907444913000346. Epub 2013 Mar 14. PMID:23519670 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Poly(A) polymerase 3D structures

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