8a4b: Difference between revisions
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==Structure of human Rep15:Rab3B_Q81L complex.== | |||
<StructureSection load='8a4b' size='340' side='right'caption='[[8a4b]], [[Resolution|resolution]] 2.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8a4b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A4B FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a4b OCA], [https://pdbe.org/8a4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a4b RCSB], [https://www.ebi.ac.uk/pdbsum/8a4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a4b ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/REP15_HUMAN REP15_HUMAN] Regulates transferrin receptor recycling from the endocytic recycling compartment.<ref>PMID:16195351</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In their GTP-bound (active) form, Rab proteins interact with effector proteins that control downstream signaling. One such Rab15 effector is Rep15, which is known to have a role in receptor recycling from the endocytic recycling compartment but otherwise remains poorly characterized. Here, we report the characterization of the Rep15:Rab15 interaction and identification of Rab3 paralogs and Rab34 as Rep15 interacting partners from a yeast two-hybrid assay. Biochemical validation of the interactions is presented and crystal structures of the Rep15:Rab3B and Rep15:Rab3C complexes provide additional mechanistic insight. We find that Rep15 adopts a globular structure that is distinct from other reported Rab15, Rab3 and Rab34 effectors. Structure-based mutagenesis experiments explain the Rep15:Rab interaction specificity. Rep15 depletion in U138MG glioblastoma cells impairs cell proliferation, cell migration and receptor recycling, underscoring the need for further clarification of the role of Rep15 in cancer. | |||
Rep15 interacts with several Rab GTPases and has a distinct fold for a Rab effector.,Rai A, Singh AK, Bleimling N, Posern G, Vetter IR, Goody RS Nat Commun. 2022 Jul 23;13(1):4262. doi: 10.1038/s41467-022-31831-1. PMID:35871249<ref>PMID:35871249</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Goody | <div class="pdbe-citations 8a4b" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
*[[Ras-related protein Rab 3D structures|Ras-related protein Rab 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Goody RS]] | |||
[[Category: Rai A]] | |||
[[Category: Vetter IR]] | |||
Latest revision as of 13:36, 1 February 2024
Structure of human Rep15:Rab3B_Q81L complex.
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