8omr: Difference between revisions
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==Human tRNA guanine transglycosylase (TGT) bound to tRNAAsp== | |||
<StructureSection load='8omr' size='340' side='right'caption='[[8omr]], [[Resolution|resolution]] 3.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8omr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8OMR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8OMR FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9DG:9-DEAZAGUANINE'>9DG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8omr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8omr OCA], [https://pdbe.org/8omr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8omr RCSB], [https://www.ebi.ac.uk/pdbsum/8omr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8omr ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/TGT_HUMAN TGT_HUMAN] Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:11255023, PubMed:20354154). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity).[UniProtKB:P28720][HAMAP-Rule:MF_03218]<ref>PMID:11255023</ref> <ref>PMID:20354154</ref> | |||
==See Also== | |||
*[[TRNA-guanine transglycosylase 3D structures|TRNA-guanine transglycosylase 3D structures]] | |||
== References == | |||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Ficner R]] | |||
[[Category: Neumann P]] | |||
[[Category: Sievers K]] | |||
[[Category: Susac L]] | |||
[[Category: Tampe R]] | |||
[[Category: Trowitzsch S]] | |||
Latest revision as of 13:41, 1 February 2024
Human tRNA guanine transglycosylase (TGT) bound to tRNAAsp
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