1nvp: Difference between revisions

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New page: left|200px<br /> <applet load="1nvp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nvp, resolution 2.10Å" /> '''HUMAN TFIIA/TBP/DNA...
 
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[[Image:1nvp.gif|left|200px]]<br />
<applet load="1nvp" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1nvp, resolution 2.10&Aring;" />
'''HUMAN TFIIA/TBP/DNA COMPLEX'''<br />


==Overview==
==HUMAN TFIIA/TBP/DNA COMPLEX==
RNA polymerase II-dependent transcription requires the assembly of a, multi-protein, preinitiation complex on core promoter elements., Transcription factor IID (TFIID) comprising the TATA box-binding protein, (TBP) and TBP-associated factors (TAFs) is responsible for promoter, recognition in this complex. Subsequent association of TFIIA and TFIIB, provides enhanced complex stability. TFIIA is required for transcriptional, stimulation by certain viral and cellular activators, and favors formation, of the preinitiation complex in the presence of repressor NC2. The X-ray, structures of human and yeast TBP/TFIIA/DNA complexes at 2.1A and 1.9A, resolution, respectively, are presented here and seen to resemble each, other closely. The interactions made by human TFIIA with TBP and DNA, within and upstream of the TATA box, including those involving water, molecules, are described and compared to the yeast structure. Of, particular interest is a previously unobserved region of TFIIA that, extends the binding interface with TBP in the yeast, but not in the human, complex, and that further elucidates biochemical and genetic results.
<StructureSection load='1nvp' size='340' side='right'caption='[[1nvp]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1nvp]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NVP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NVP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nvp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nvp OCA], [https://pdbe.org/1nvp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nvp RCSB], [https://www.ebi.ac.uk/pdbsum/1nvp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nvp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TF2AA_HUMAN TF2AA_HUMAN] TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.<ref>PMID:11030333</ref> <ref>PMID:16537915</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nv/1nvp_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nvp ConSurf].
<div style="clear:both"></div>


==Disease==
==See Also==
Known diseases associated with this structure: Huntington disease-like-4 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600075 600075]], Parkinson disease OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600075 600075]], Spinocerebellar ataxia 17 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600075 600075]]
*[[TATA-binding protein 3D structures|TATA-binding protein 3D structures]]
 
*[[Transcription initiation factors 3D structures|Transcription initiation factors 3D structures]]
==About this Structure==
== References ==
1NVP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NVP OCA].
<references/>
 
__TOC__
==Reference==
</StructureSection>
Novel interactions between the components of human and yeast TFIIA/TBP/DNA complexes., Bleichenbacher M, Tan S, Richmond TJ, J Mol Biol. 2003 Sep 26;332(4):783-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12972251 12972251]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Bleichenbacher, M.]]
[[Category: Bleichenbacher M]]
[[Category: Richmond, T.J.]]
[[Category: Richmond TJ]]
[[Category: Tan, S.]]
[[Category: Tan S]]
[[Category: complex]]
[[Category: dna]]
[[Category: transcription regulation]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:26:10 2007''

Latest revision as of 07:59, 14 February 2024

HUMAN TFIIA/TBP/DNA COMPLEX

1nvp, resolution 2.10Å

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