7tmt

V-ATPase from Saccharomyces cerevisiae, State 3

spinqualitylabelsall models 7tmt, resolution 3.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7tmt is a 18 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.8Å Ligands: ADP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B3LH69_YEAS1 Publication Abstract from PubMed Vacuolar-type ATPases (V-ATPases) are rotary enzymes that acidify intracellular compartments in eukaryotic cells. These multi-subunit complexes consist of a cytoplasmic V(1) region that hydrolyzes ATP and a membrane-embedded V(O) region that transports protons. V-ATPase activity is regulated by reversible dissociation of the two regions, with the isolated V(1) and V(O) complexes becoming autoinhibited on disassembly and subunit C subsequently detaching from V(1). In yeast, assembly of the V(1) and V(O) regions is mediated by the regulator of the ATPase of vacuoles and endosomes (RAVE) complex through an unknown mechanism. We used cryogenic-electron microscopy of yeast V-ATPase to determine structures of the intact enzyme, the dissociated but complete V(1) complex and the V(1) complex lacking subunit C. On separation, V(1) undergoes a dramatic conformational rearrangement, with its rotational state becoming incompatible for reassembly with V(O). Loss of subunit C allows V(1) to match the rotational state of V(O), suggesting how RAVE could reassemble V(1) and V(O) by recruiting subunit C. Coordinated conformational changes in the V(1) complex during V-ATPase reversible dissociation.,Vasanthakumar T, Keon KA, Bueler SA, Jaskolka MC, Rubinstein JL Nat Struct Mol Biol. 2022 May;29(5):430-439. doi: 10.1038/s41594-022-00757-z. , Epub 2022 Apr 25. PMID:35469063 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also ATPase 3D structures

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