9bt4: Difference between revisions
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New page: '''Unreleased structure''' The entry 9bt4 is ON HOLD Authors: Catlin, D., Nair, S.J. Description: Pyruvate:Ferredoxin Oxidoreductase from Methanosarcina acetivorans [[Category: Unrelea... |
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==Pyruvate:Ferredoxin Oxidoreductase from Methanosarcina acetivorans== | |||
<StructureSection load='9bt4' size='340' side='right'caption='[[9bt4]], [[Resolution|resolution]] 1.92Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9bt4]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_acetivorans_C2A Methanosarcina acetivorans C2A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9BT4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9BT4 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9bt4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9bt4 OCA], [https://pdbe.org/9bt4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9bt4 RCSB], [https://www.ebi.ac.uk/pdbsum/9bt4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9bt4 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8TUN5_METAC Q8TUN5_METAC] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Enzymes of the 2-oxoacid:ferredoxin oxidoreductase (OFOR) superfamily catalyze the reversible oxidation of 2-oxoacids to acyl-coenzyme A esters and carbon dioxide (CO(2))using ferredoxin or flavodoxin as the redox partner. Although members of the family share primary sequence identity, a variety of domain and subunit arrangements are known. Here, we characterize the structure of a four-subunit family member: the pyruvate:ferredoxin oxidoreductase (PFOR) from the methane producing archaeon Methanosarcina acetivorans (MaPFOR). The 1.92 A resolution crystal structure of MaPFOR shows a protein fold like those of single- or two-subunit PFORs that function in 2-oxoacid oxidation, including the location of the requisite thiamine pyrophosphate (TPP), and three [4Fe-4S] clusters. Of note, MaPFOR typically functions in the CO(2) reductive direction, and structural comparisons to the pyruvate oxidizing PFORs show subtle differences in several regions of catalytical relevance. These studies provide a framework that may shed light on the biochemical mechanisms used to facilitate reductive pyruvate synthesis. | |||
Structural organization of pyruvate: ferredoxin oxidoreductase from the methanogenic archaeon Methanosarcina acetivorans.,Cossu M, Catlin D, Elliott SJ, Metcalf WW, Nair SK Structure. 2024 Aug 30:S0969-2126(24)00328-9. doi: 10.1016/j.str.2024.08.011. PMID:39265575<ref>PMID:39265575</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Catlin | <div class="pdbe-citations 9bt4" style="background-color:#fffaf0;"></div> | ||
[[Category: Nair | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Methanosarcina acetivorans C2A]] | |||
[[Category: Catlin D]] | |||
[[Category: Nair SJ]] | |||
Latest revision as of 07:07, 9 October 2024
Pyruvate:Ferredoxin Oxidoreductase from Methanosarcina acetivorans
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