9y1s: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: '''Unreleased structure''' The entry 9y1s is ON HOLD until Paper Publication Authors: Chen, K., Chen, Y., DeGrado, W.F., Huang, B. Description: Rhobin9, de novo rhodamine binder, apo f... |
No edit summary |
||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Rhobin9, de novo rhodamine binder, apo form== | |||
<StructureSection load='9y1s' size='340' side='right'caption='[[9y1s]], [[Resolution|resolution]] 1.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9y1s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9Y1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9Y1S FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9y1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9y1s OCA], [https://pdbe.org/9y1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9y1s RCSB], [https://www.ebi.ac.uk/pdbsum/9y1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9y1s ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Fluorescent imaging in live cells is a cornerstone of life sciences. While natural fluorescent proteins have been engineered to enhance individual features, no existing tag combines ideal properties into a single system: high brightness, reversible binding, compact size, and stability across diverse conditions. Here, we achieve this through de novo design of rhodamine binders (Rhobin). To harness the broad repertoire of rhodamine fluorophores, we developed a generalizable design strategy for a pan-rhodamine binder compatible with diverse wavelengths and applications. Rhobin enables live- and fixed-cell imaging of various subcellular targets in mammalian cells, showing brightness surpassing existing tags. Its reversible fluorophore binding supports super-resolution stimulated emission depletion (STED) and live-cell single-molecule imaging for extended durations compared with HaloTag. Beyond conventional systems, Rhobin enables live imaging of the extremophile Sulfolobus acidocaldarius at 75 degrees C, previously inaccessible with current tags. Together, these results establish Rhobin as a versatile platform for next-generation imaging and biosensor design. | |||
De novo pan-rhodamine binders for fluorescence microscopy from mammalian cells to extremophiles.,Chen Y, Yserentant K, Hong K, Chen K, Kuang Y, Picardo RS, Bhowmick A, Charles-Orszag A, Lord SJ, Lu L, Hou K, Mann SI, Bhattacharya S, Horst M, Grimm JB, Lavis LD, Mullins RD, DeGrado WF, Huang B Cell. 2026 Sep 1:S0092-8674(26)00934-7. doi: 10.1016/j.cell.2026.08.007. PMID:42679818<ref>PMID:42679818</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 9y1s" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: Chen | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Chen K]] | |||
[[Category: Chen Y]] | |||
[[Category: DeGrado WF]] | |||
[[Category: Huang B]] | |||
