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New page: left|200px<br /> <applet load="2a26" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a26, resolution 1.20Å" /> '''Crystal structure o...
 
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[[Image:2a26.gif|left|200px]]<br />
<applet load="2a26" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2a26, resolution 1.20&Aring;" />
'''Crystal structure of the N-terminal, dimerization domain of Siah Interacting Protein'''<br />


==Overview==
==Crystal structure of the N-terminal, dimerization domain of Siah Interacting Protein==
Siah1 is the central component of a multiprotein E3 ubiquitin ligase, complex that targets beta-catenin for destruction in response to p53, activation. The E3 complex comprises, in addition to Siah1, Siah-interacting protein (SIP), the adaptor protein Skp1, and the F-box, protein Ebi. Here we show that SIP engages Siah1 by means of two elements, both of which are required for mediating beta-catenin destruction in, cells. An N-terminal dimerization domain of SIP sits across the, saddle-shaped upper surface of Siah1, with two extended legs packing, against the sides of Siah1 by means of a consensus PXAXVXP motif that is, common to a family of Siah-binding proteins. The C-terminal domain of SIP, which binds to Skp1, protrudes from the lower surface of Siah1, and we, propose that this surface provides the scaffold for bringing substrate and, the E2 enzyme into apposition in the functional complex.
<StructureSection load='2a26' size='340' side='right'caption='[[2a26]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
 
== Structural highlights ==
==Disease==
<table><tr><td colspan='2'>[[2a26]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A26 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A26 FirstGlance]. <br>
Known disease associated with this structure: Mowat-Wilson syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=605802 605802]]
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==About this Structure==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a26 OCA], [https://pdbe.org/2a26 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a26 RCSB], [https://www.ebi.ac.uk/pdbsum/2a26 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a26 ProSAT]</span></td></tr>
2A26 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 and CXS as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2A26 OCA].  
</table>
 
== Function ==
==Reference==
[https://www.uniprot.org/uniprot/CYBP_HUMAN CYBP_HUMAN] May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).<ref>PMID:16085652</ref>
Structural analysis of Siah1-Siah-interacting protein interactions and insights into the assembly of an E3 ligase multiprotein complex., Santelli E, Leone M, Li C, Fukushima T, Preece NE, Olson AJ, Ely KR, Reed JC, Pellecchia M, Liddington RC, Matsuzawa S, J Biol Chem. 2005 Oct 7;280(40):34278-87. Epub 2005 Aug 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16085652 16085652]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Ely, K.R.]]
[[Category: Ely KR]]
[[Category: Fukushima, T.]]
[[Category: Fukushima T]]
[[Category: Leone, M.]]
[[Category: Leone M]]
[[Category: Li, C.]]
[[Category: Li C]]
[[Category: Liddington, R.C.]]
[[Category: Liddington RC]]
[[Category: Matsuzawa, S.]]
[[Category: Matsuzawa S]]
[[Category: Olson, A.J.]]
[[Category: Olson AJ]]
[[Category: Pellecchia, M.]]
[[Category: Pellecchia M]]
[[Category: Preece, N.E.]]
[[Category: Preece NE]]
[[Category: Reed, J.C.]]
[[Category: Reed JC]]
[[Category: Santelli, E.]]
[[Category: Santelli E]]
[[Category: CXS]]
[[Category: SO4]]
[[Category: dimerization]]
[[Category: helical hairpin]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:45:00 2007''

Latest revision as of 09:10, 14 February 2024

Crystal structure of the N-terminal, dimerization domain of Siah Interacting Protein

2a26, resolution 1.20Å

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