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New page: left|200px<br /> <applet load="1ee5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ee5, resolution 2.4Å" /> '''YEAST KARYOPHERIN (I...
 
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[[Image:1ee5.gif|left|200px]]<br />
<applet load="1ee5" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ee5, resolution 2.4&Aring;" />
'''YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A NUCLEOPLASMIN NLS PEPTIDE'''<br />


==Overview==
==YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A NUCLEOPLASMIN NLS PEPTIDE==
BACKGROUND: Karyopherin alpha (importin alpha) is an adaptor molecule that, recognizes proteins containing nuclear localization signals (NLSs). The, prototypical NLS that is able to bind to karyopherin alpha is that of the, SV40 T antigen, and consists of a short positively charged sequence motif., Distinct classes of NLSs (monopartite and bipartite) have been identified, that are only partly conserved with respect to one another but are, nevertheless recognized by the same receptor. RESULTS: We report the, crystal structures of two peptide complexes of yeast karyopherin alpha, (Kapalpha): one with a human c-myc NLS peptide, determined at 2.1 A, resolution, and one with a Xenopus nucleoplasmin NLS peptide, determined, at 2.4 A resolution. Analysis of these structures reveals the determinants, of specificity for the binding of a relatively hydrophobic monopartite NLS, and of a bipartite NLS peptide. The peptides bind Kapalpha in its extended, surface groove, which presents a modular array of tandem binding pockets, for amino acid residues. CONCLUSIONS: Monopartite and bipartite NLSs bind, to a different number of amino acid binding pockets and make different, interactions within them. The relatively hydrophobic monopartite c-myc NLS, binds extensively at a few binding pockets in a similar manner to that of, the SV40 T antigen NLS. In contrast, the bipartite nucleoplasmin NLS, engages the whole array of pockets with individually more limited but, overall more abundant interactions, which include the NLS two basic, clusters and the backbone of its non-conserved linker region. Versatility, in the specific recognition of NLSs relies on the modular.
<StructureSection load='1ee5' size='340' side='right'caption='[[1ee5]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ee5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. The January 2007 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Importins''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2007_1 10.2210/rcsb_pdb/mom_2007_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EE5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EE5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ee5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ee5 OCA], [https://pdbe.org/1ee5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ee5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ee5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ee5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IMA1_YEAST IMA1_YEAST] Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Promotes docking of import substrates to the nuclear envelope. Seems to act as a cytosolic receptor for both simple and bipartite NLS motifs (By similarity).<ref>PMID:7565597</ref> <ref>PMID:10913188</ref> <ref>PMID:21075847</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ee/1ee5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ee5 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1EE5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. The following page contains interesting information on the relation of 1EE5 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb85_1.html Importins]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EE5 OCA].
*[[Importin|Importin]]
 
*[[Importin 3D structures|Importin 3D structures]]
==Reference==
*[[Nucleoplasmin|Nucleoplasmin]]
Crystallographic analysis of the specific yet versatile recognition of distinct nuclear localization signals by karyopherin alpha., Conti E, Kuriyan J, Structure. 2000 Mar 15;8(3):329-38. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10745017 10745017]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Importins]]
[[Category: Importins]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Xenopus laevis]]
[[Category: Xenopus laevis]]
[[Category: Conti, E.]]
[[Category: Conti E]]
[[Category: armadillo repeat]]
 
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Latest revision as of 07:01, 7 February 2024

YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A NUCLEOPLASMIN NLS PEPTIDE

1ee5, resolution 2.40Å

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