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New page: left|200px<br /> <applet load="2a7u" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a7u" /> '''NMR solution structure of the E.coli F-ATPa...
 
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[[Image:2a7u.gif|left|200px]]<br />
<applet load="2a7u" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2a7u" />
'''NMR solution structure of the E.coli F-ATPase delta subunit N-terminal domain in complex with alpha subunit N-terminal 22 residues'''<br />


==Overview==
==NMR solution structure of the E.coli F-ATPase delta subunit N-terminal domain in complex with alpha subunit N-terminal 22 residues==
A critical point of interaction between F(1) and F(0) in the bacterial, F(1)F(0)-ATP synthase is formed by the alpha and delta subunits. Previous, work has shown that the N-terminal domain (residues 3-105) of the delta, subunit forms a 6 alpha-helix bundle [Wilkens, S., Dunn, S. D., Chandler, J., Dahlquist, F. W., and Capaldi, R. A. (1997) Nat. Struct. Biol. 4, 198-201] and that the majority of the binding energy between delta and, F(1) is provided by the interaction between the N-terminal 22 residues of, the alpha- and N-terminal domain of the delta subunit [Weber, J., Muharemagic, A., Wilke-Mounts, S., and Senior, A. E. (2003) J. Biol. Chem., 278, 13623-13626]. We have now analyzed a 1:1 complex of the delta-subunit, N-terminal domain and a peptide comprising the N-terminal 22 residues of, the alpha subunit by heteronuclear protein NMR spectroscopy. A comparison, of the chemical-shift values of delta-subunit residues with and without, alpha N-terminal peptide bound indicates that the binding interface on the, N-terminal domain of the delta subunit is formed by alpha helices I and V., NOE cross-peak patterns in 2D (12)C/(12)C-filtered NOESY spectra of the, (13)C-labeled delta-subunit N-terminal domain in complex with unlabeled, peptide verify that residues 8-18 in the alpha-subunit N-terminal peptide, are folded as an alpha helix when bound to delta N-terminal domain. On the, basis of intermolecular contacts observed in (12)C/(13)C-filtered NOESY, experiments, we describe structural details of the interaction of the, delta-subunit N-terminal domain with the alpha-subunit N-terminal alpha, helix.
<StructureSection load='2a7u' size='340' side='right'caption='[[2a7u]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2a7u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. The December 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''ATP Synthase''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_12 10.2210/rcsb_pdb/mom_2005_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A7U FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7u OCA], [https://pdbe.org/2a7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a7u RCSB], [https://www.ebi.ac.uk/pdbsum/2a7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a7u ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ATPA_ECO57 ATPA_ECO57] Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a7/2a7u_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a7u ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2A7U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The following page contains interesting information on the relation of 2A7U with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb72_1.html ATP Synthase]]. Active as [http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2A7U OCA].
*[[ATPase 3D structures|ATPase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structural characterization of the interaction of the delta and alpha subunits of the Escherichia coli F1F0-ATP synthase by NMR spectroscopy., Wilkens S, Borchardt D, Weber J, Senior AE, Biochemistry. 2005 Sep 6;44(35):11786-94. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16128580 16128580]
[[Category: ATP Synthase]]
[[Category: ATP Synthase]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: H(+)-transporting two-sector ATPase]]
[[Category: Escherichia coli O157:H7]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Borchardt, D.]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Senior, A.E.]]
[[Category: Borchardt D]]
[[Category: Weber, J.]]
[[Category: Senior AE]]
[[Category: Wilkens, S.]]
[[Category: Weber J]]
[[Category: alpha helix bundle]]
[[Category: Wilkens S]]
 
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Latest revision as of 06:37, 1 May 2024

NMR solution structure of the E.coli F-ATPase delta subunit N-terminal domain in complex with alpha subunit N-terminal 22 residues

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