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New page: left|200px<br /><applet load="1aro" size="450" color="white" frame="true" align="right" spinBox="true" caption="1aro, resolution 2.8Å" /> '''T7 RNA POLYMERASE COM...
 
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[[Image:1aro.jpg|left|200px]]<br /><applet load="1aro" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1aro, resolution 2.8&Aring;" />
'''T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME'''<br />


==Overview==
==T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME==
The T7 RNA polymerase-T7 lysozyme complex regulates phage gene expression, during infection of Escherichia coli. The 2.8 A crystal structure of the, complex reveals that lysozyme binds at a site remote from the polymerase, active site, suggesting an indirect mechanism of inhibition. Comparison of, the T7 RNA polymerase structure with that of the homologous pol I family, of DNA polymerases reveals identities in the catalytic site but also, differences specific to RNA polymerase function. The structure of T7 RNA, polymerase presented here differs significantly from a previously, published structure. Sequence similarities between phage RNA polymerases, and those from mitochondria and chloroplasts, when interpreted in the, context of our revised model of T7 RNA polymerase, suggest a conserved, fold.
<StructureSection load='1aro' size='340' side='right'caption='[[1aro]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1aro]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ARO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ARO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aro OCA], [https://pdbe.org/1aro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aro RCSB], [https://www.ebi.ac.uk/pdbsum/1aro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aro ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RPOL_BPT7 RPOL_BPT7] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ar/1aro_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aro ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1ARO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] with HG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ARO OCA].
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
 
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
==Reference==
__TOC__
Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme., Jeruzalmi D, Steitz TA, EMBO J. 1998 Jul 15;17(14):4101-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9670025 9670025]
</StructureSection>
[[Category: Bacteriophage t7]]
[[Category: Escherichia phage T7]]
[[Category: DNA-directed RNA polymerase]]
[[Category: Large Structures]]
[[Category: Protein complex]]
[[Category: Jeruzalmi D]]
[[Category: Jeruzalmi, D.]]
[[Category: Steitz T]]
[[Category: Steitz, T.]]
[[Category: HG]]
[[Category: complex (polymerase/hydrolase)]]
[[Category: dna-directed rna polymerase]]
[[Category: glycosidase]]
[[Category: hydrolase]]
[[Category: transcription]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:04:44 2007''

Latest revision as of 06:32, 7 February 2024

T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME

1aro, resolution 2.80Å

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