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New page: left|200px<br /><applet load="1dj0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dj0, resolution 1.50Å" /> '''THE CRYSTAL STRUCTUR...
 
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[[Image:1dj0.jpg|left|200px]]<br /><applet load="1dj0" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1dj0, resolution 1.50&Aring;" />
'''THE CRYSTAL STRUCTURE OF E. COLI PSEUDOURIDINE SYNTHASE I AT 1.5 ANGSTROM RESOLUTION'''<br />


==Overview==
==THE CRYSTAL STRUCTURE OF E. COLI PSEUDOURIDINE SYNTHASE I AT 1.5 ANGSTROM RESOLUTION==
Pseudouridine synthases catalyze the isomerization of specific uridines to, pseudouridine in a variety of RNAs, yet the basis for recognition of the, RNA sites or how they catalyze this reaction is unknown. The crystal, structure of pseudouridine synthase I from Escherichia coli, which, for, example, modifies positions 38, 39 and/or 40 in tRNA, reveals a dimeric, protein that contains two positively charged, RNA-binding clefts along the, surface of the protein. Each cleft contains a highly conserved aspartic, acid located at its center. The structural domains have a topological, similarity to those of other RNA-binding proteins, though the mode of, interaction with tRNA appears to be unique. The structure suggests that a, dimeric enzyme is required for binding transfer RNA and subsequent, pseudouridine formation.
<StructureSection load='1dj0' size='340' side='right'caption='[[1dj0]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1dj0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DJ0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dj0 OCA], [https://pdbe.org/1dj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dj0 RCSB], [https://www.ebi.ac.uk/pdbsum/1dj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dj0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TRUA_ECOLI TRUA_ECOLI] Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.<ref>PMID:17466622</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dj/1dj0_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dj0 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1DJ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DJ0 OCA].
*[[Guide-independent Pseudouridine synthase|Guide-independent Pseudouridine synthase]]
 
*[[Pseudouridine synthase 3D structures|Pseudouridine synthase 3D structures]]
==Reference==
== References ==
The structural basis for tRNA recognition and pseudouridine formation by pseudouridine synthase I., Foster PG, Huang L, Santi DV, Stroud RM, Nat Struct Biol. 2000 Jan;7(1):23-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10625422 10625422]
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Pseudouridylate synthase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Foster PG]]
[[Category: Foster, P.G.]]
[[Category: Huang L]]
[[Category: Huang, L.]]
[[Category: Santi DV]]
[[Category: Santi, D.V.]]
[[Category: Stroud RM]]
[[Category: Stroud, R.M.]]
[[Category: CL]]
[[Category: alpha/beta fold]]
[[Category: rna-binding motif]]
[[Category: rna-modifying enzyme]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:19:06 2007''

Latest revision as of 06:54, 7 February 2024

THE CRYSTAL STRUCTURE OF E. COLI PSEUDOURIDINE SYNTHASE I AT 1.5 ANGSTROM RESOLUTION

1dj0, resolution 1.50Å

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