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New page: left|200px<br /><applet load="1l8q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l8q, resolution 2.700Å" /> '''CRYSTAL STRUCTURE O...
 
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[[Image:1l8q.gif|left|200px]]<br /><applet load="1l8q" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1l8q, resolution 2.700&Aring;" />
'''CRYSTAL STRUCTURE OF DNA REPLICATION INITIATION FACTOR'''<br />


==Overview==
==CRYSTAL STRUCTURE OF DNA REPLICATION INITIATION FACTOR==
The initiation of DNA replication is a key event in the cell cycle of all, organisms. In bacteria, replication initiation occurs at specific origin, sequences that are recognized and processed by an oligomeric complex of, the initiator protein DnaA. We have determined the structure of the, conserved core of the Aquifex aeolicus DnaA protein to 2.7 A resolution., The protein comprises an AAA+ nucleotide-binding fold linked through a, long, helical connector to an all-helical DNA-binding domain. The, structure serves as a template for understanding the physical consequences, of a variety of DnaA mutations, and conserved motifs in the protein, suggest how two critical aspects of origin processing, DNA binding and, homo-oligomerization, are mediated. The spatial arrangement of these, motifs in DnaA is similar to that of the eukaryotic-like archaeal, replication initiation factor Cdc6/Orc1, demonstrating that mechanistic, elements of origin processing may be conserved across bacterial, archaeal, and eukaryotic domains of life.
<StructureSection load='1l8q' size='340' side='right'caption='[[1l8q]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1l8q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L8Q FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l8q OCA], [https://pdbe.org/1l8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l8q RCSB], [https://www.ebi.ac.uk/pdbsum/1l8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l8q ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DNAA_AQUAE DNAA_AQUAE] Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l8/1l8q_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l8q ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1L8Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1L8Q OCA].
*[[DnaA|DnaA]]
 
__TOC__
==Reference==
</StructureSection>
The structure of bacterial DnaA: implications for general mechanisms underlying DNA replication initiation., Erzberger JP, Pirruccello MM, Berger JM, EMBO J. 2002 Sep 16;21(18):4763-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12234917 12234917]
[[Category: Aquifex aeolicus]]
[[Category: Aquifex aeolicus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Berger, J.M.]]
[[Category: Berger JM]]
[[Category: Erzberger, J.P.]]
[[Category: Erzberger JP]]
[[Category: Pirruccello, M.M.]]
[[Category: Pirruccello MM]]
[[Category: ADP]]
[[Category: MG]]
[[Category: aaa+]]
[[Category: dna binding]]
[[Category: helix-turn-helix]]
[[Category: nucleotide-binding]]
[[Category: replication initiation]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 20:22:39 2007''

Latest revision as of 08:07, 3 April 2024

CRYSTAL STRUCTURE OF DNA REPLICATION INITIATION FACTOR

1l8q, resolution 2.70Å

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