2pi5: Difference between revisions

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{{Seed}}
[[Image:2pi5.png|left|200px]]


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==T7 RNA polymerase complexed with a phi10 promoter==
The line below this paragraph, containing "STRUCTURE_2pi5", creates the "Structure Box" on the page.
<StructureSection load='2pi5' size='340' side='right'caption='[[2pi5]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2pi5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PI5 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
-->
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pi5 OCA], [https://pdbe.org/2pi5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pi5 RCSB], [https://www.ebi.ac.uk/pdbsum/2pi5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pi5 ProSAT]</span></td></tr>
{{STRUCTURE_2pi5| PDB=2pi5 |  SCENE= }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/RPOL_BPT7 RPOL_BPT7] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pi/2pi5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pi5 ConSurf].
<div style="clear:both"></div>


===T7 RNA polymerase complexed with a phi10 promoter===
==See Also==
 
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
 
__TOC__
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</StructureSection>
The line below this paragraph, {{ABSTRACT_PUBMED_17512007}}, adds the Publication Abstract to the page
[[Category: Escherichia phage T7]]
(as it appears on PubMed at http://www.pubmed.gov), where 17512007 is the PubMed ID number.
[[Category: Large Structures]]
-->
[[Category: Kennedy WP]]
{{ABSTRACT_PUBMED_17512007}}
[[Category: Momand JR]]
 
[[Category: Yin YW]]
==About this Structure==
2PI5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PI5 OCA].
 
==Reference==
Mechanism for de novo RNA synthesis and initiating nucleotide specificity by t7 RNA polymerase., Kennedy WP, Momand JR, Yin YW, J Mol Biol. 2007 Jul 6;370(2):256-68. Epub 2007 Mar 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17512007 17512007]
[[Category: DNA-directed RNA polymerase]]
[[Category: Enterobacteria phage t7]]
[[Category: Single protein]]
[[Category: Kennedy, W P.]]
[[Category: Momand, J R.]]
[[Category: Yin, Y W.]]
[[Category: Initiating nucleoite]]
[[Category: T7 rna polymerase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:29:50 2008''

Latest revision as of 09:09, 21 February 2024

T7 RNA polymerase complexed with a phi10 promoter

2pi5, resolution 2.90Å

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