1zhs: Difference between revisions

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{{Seed}}
[[Image:1zhs.png|left|200px]]


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==Crystal structure of MVL bound to Man3GlcNAc2==
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<StructureSection load='1zhs' size='340' side='right'caption='[[1zhs]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1zhs]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Microcystis_viridis Microcystis viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZHS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
{{STRUCTURE_1zhs|  PDB=1zhs  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zhs OCA], [https://pdbe.org/1zhs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zhs RCSB], [https://www.ebi.ac.uk/pdbsum/1zhs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zhs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MVL_MICVR MVL_MICVR] Carbohydrate-binding protein that binds oligomannosides such as Man(6)GlcNAc(2) with sub-micromolar affinities. The specificity of MVL is unique in that its minimal target comprises the Man-alpha-(1->6)-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc tetrasaccharide core (Man(2)A) found in N-linked oligomannosides. Displays hemagglutininating activity on rabbit, horse and hen erythrocytes. This activity is inhibited by yeast mannan. Does not bind mono- and disachharides. Inhibits HIV-1 envelope-mediated cell fusion at nanomolar concentrations through carbohydrate-mediated interactions with high-mannose residues on the surface of the HIV envelope glycoprotein gp120.  Unexpectedly for a lectin, one of the 2 oligomannose binding sites of MVL can catalyze the cleavage of chitin fragments (such as chitotriose, i.e. GlcNAc(3) or GlcNAc-beta-(1->4)-GlcNAcbeta-(1->4)-GlcNAc, and chitotetraose, i.e. GlcNAc(4)) to GlcNAc. This weak glycosidase activity is restricted to the C-terminal carbohydrate-binding site. Does not cleave Man(3)GlcNAc(2) or the tetrasaccharide Man(2)A.


===Crystal structure of MVL bound to Man3GlcNAc2===
==See Also==
 
*[[Mannose-binding protein|Mannose-binding protein]]
 
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[[Category: Large Structures]]
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{{ABSTRACT_PUBMED_15937331}}
 
==About this Structure==
1ZHS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Microcystis_viridis Microcystis viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZHS OCA].
 
==Reference==
Crystal structures of the HIV-1 inhibitory cyanobacterial protein MVL free and bound to Man3GlcNAc2: structural basis for specificity and high-affinity binding to the core pentasaccharide from n-linked oligomannoside., Williams DC Jr, Lee JY, Cai M, Bewley CA, Clore GM, J Biol Chem. 2005 Aug 12;280(32):29269-76. Epub 2005 Jun 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15937331 15937331]
[[Category: Microcystis viridis]]
[[Category: Microcystis viridis]]
[[Category: Single protein]]
[[Category: Bewley CA]]
[[Category: Bewley, C A.]]
[[Category: Cai M]]
[[Category: Cai, M.]]
[[Category: Clore GM]]
[[Category: Clore, G M.]]
[[Category: Lee JY]]
[[Category: Lee, J Y.]]
[[Category: Williams DC]]
[[Category: Williams, D C.]]
[[Category: Carbohydrate binding]]
[[Category: Hiv-1]]
[[Category: Mvl]]
 
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Retrieved from "https://proteopedia.org/1zhs"