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New page: left|200px<br /><applet load="1tip" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tip, resolution 2.2Å" /> '''THE BISPHOSPHATASE DO...
 
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[[Image:1tip.jpg|left|200px]]<br /><applet load="1tip" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1tip, resolution 2.2&Aring;" />
'''THE BISPHOSPHATASE DOMAIN OF THE BIFUNCTIONAL RAT LIVER 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE'''<br />


==Overview==
==THE BISPHOSPHATASE DOMAIN OF THE BIFUNCTIONAL RAT LIVER 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE==
The crystal structure of the fructose-2,6-bisphosphatase domain trapped, during the reaction reveal a phosphorylated His 258, and a water molecule, immobilized by the product, fructose-6-phosphate. The geometry suggests, that the dephosphorylation step requires prior removal of the product for, an 'associative in-line' phosphoryl transfer to the catalytic water.
<StructureSection load='1tip' size='340' side='right'caption='[[1tip]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1tip]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TIP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F6P:FRUCTOSE-6-PHOSPHATE'>F6P</scene>, <scene name='pdbligand=NEP:N1-PHOSPHONOHISTIDINE'>NEP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tip OCA], [https://pdbe.org/1tip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tip RCSB], [https://www.ebi.ac.uk/pdbsum/1tip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tip ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/F261_RAT F261_RAT] Synthesis and degradation of fructose 2,6-bisphosphate.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ti/1tip_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tip ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of the fructose-2,6-bisphosphatase domain trapped during the reaction reveal a phosphorylated His 258, and a water molecule immobilized by the product, fructose-6-phosphate. The geometry suggests that the dephosphorylation step requires prior removal of the product for an 'associative in-line' phosphoryl transfer to the catalytic water.


==About this Structure==
Crystal structure of a trapped phosphoenzyme during a catalytic reaction.,Lee YH, Olson TW, Ogata CM, Levitt DG, Banaszak LJ, Lange AJ Nat Struct Biol. 1997 Aug;4(8):615-8. PMID:9253407<ref>PMID:9253407</ref>
1TIP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with F6P as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Fructose-2,6-bisphosphate_2-phosphatase Fructose-2,6-bisphosphate 2-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.46 3.1.3.46] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TIP OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystal structure of a trapped phosphoenzyme during a catalytic reaction., Lee YH, Olson TW, Ogata CM, Levitt DG, Banaszak LJ, Lange AJ, Nat Struct Biol. 1997 Aug;4(8):615-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9253407 9253407]
</div>
[[Category: Fructose-2,6-bisphosphate 2-phosphatase]]
<div class="pdbe-citations 1tip" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Single protein]]
[[Category: Banaszak LJ]]
[[Category: Banaszak, L.J.]]
[[Category: Lange AJ]]
[[Category: Lange, A.J.]]
[[Category: Lee Y-H]]
[[Category: Lee, Y.H.]]
[[Category: Levitt DG]]
[[Category: Levitt, D.G.]]
[[Category: Ogata CM]]
[[Category: Ogata, C.M.]]
[[Category: Olson TW]]
[[Category: Olson, T.W.]]
[[Category: F6P]]
[[Category: alternative splicing]]
[[Category: atp-binding]]
[[Category: hydrolase]]
[[Category: kinase]]
[[Category: multifunctional enzyme]]
[[Category: multigene family]]
[[Category: phosphorylation]]
[[Category: transferase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:18:08 2007''

Latest revision as of 22:15, 26 March 2025

THE BISPHOSPHATASE DOMAIN OF THE BIFUNCTIONAL RAT LIVER 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE

1tip, resolution 2.20Å

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