2vvs: Difference between revisions

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{{Seed}}
[[Image:2vvs.png|left|200px]]


<!--
==BtGH84 structure in complex with PUGNAc==
The line below this paragraph, containing "STRUCTURE_2vvs", creates the "Structure Box" on the page.
<StructureSection load='2vvs' size='340' side='right'caption='[[2vvs]], [[Resolution|resolution]] 2.24&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2vvs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron_VPI-5482 Bacteroides thetaiotaomicron VPI-5482]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VVS FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.24&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OAN:O-(2-ACETAMIDO-2-DEOXY+D-GLUCOPYRANOSYLIDENE)+AMINO-N-PHENYLCARBAMATE'>OAN</scene></td></tr>
{{STRUCTURE_2vvs|  PDB=2vvs  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vvs OCA], [https://pdbe.org/2vvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vvs RCSB], [https://www.ebi.ac.uk/pdbsum/2vvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vvs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/OGA_BACTN OGA_BACTN] Biological function unknown. Capable of hydrolyzing the glycosidic link of O-GlcNAcylated proteins.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vv/2vvs_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vvs ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The O-GlcNAc post-translational modification is considered to act as a sensor of nutrient flux through the hexosamine biosynthetic pathway. A cornerstone of this hypothesis is that global elevation of protein O-GlcNAc levels, typically induced with the non-selective O-GlcNAcase inhibitor PUGNAc (O-(2-acetamido-2-deoxy-D-glycopyranosylidene) amino-N-phenylcarbamate), causes insulin resistance in adipocytes. Here we address the potential link between elevated O-GlcNAc and insulin resistance by using a potent and selective inhibitor of O-GlcNAcase (NButGT (1,2-dideoxy-2'-propyl-alpha-D-glucopyranoso-[2,1-D]-Delta 2'-thiazoline), 1200-fold selectivity). A comparison of the structures of a bacterial homologue of O-GlcNAcase in complex with PUGNAc or NButGT reveals that these inhibitors bind to the same region of the active site, underscoring the competitive nature of their inhibition of O-GlcNAcase and the molecular basis of selectivity. Treating 3T3-L1 adipocytes with NButGT induces rapid increases in global O-GlcNAc levels, but strikingly, NButGT treatment does not replicate the insulin desensitizing effects of the non-selective O-GlcNAcase inhibitor PUGNAc. Consistent with these observations, NButGT also does not recapitulate the impaired insulin-mediated phosphorylation of Akt that is induced by treatment with PUGNAc. Collectively, these results suggest that increases in global levels of O-GlcNAc-modified proteins of cultured adipocytes do not, on their own, cause insulin resistance.


===BTGH84 STRUCTURE IN COMPLEX WITH PUGNAC===
Elevation of global O-GlcNAc levels in 3T3-L1 adipocytes by selective inhibition of O-GlcNAcase does not induce insulin resistance.,Macauley MS, Bubb AK, Martinez-Fleites C, Davies GJ, Vocadlo DJ J Biol Chem. 2008 Dec 12;283(50):34687-95. Epub 2008 Oct 8. PMID:18842583<ref>PMID:18842583</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2vvs" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_18842583}}, adds the Publication Abstract to the page
*[[Beta-Hexosaminidase|Beta-Hexosaminidase]]
(as it appears on PubMed at http://www.pubmed.gov), where 18842583 is the PubMed ID number.
*[[Beta-Hexosaminidase 3D structures|Beta-Hexosaminidase 3D structures]]
-->
*[[O-GlcNAcase|O-GlcNAcase]]
{{ABSTRACT_PUBMED_18842583}}
== References ==
 
<references/>
==About this Structure==
__TOC__
2VVS is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VVS OCA].
</StructureSection>
 
[[Category: Bacteroides thetaiotaomicron VPI-5482]]
==Reference==
[[Category: Large Structures]]
Elevation of Global O-GlcNAc Levels in 3T3-L1 Adipocytes by Selective Inhibition of O-GlcNAcase Does Not Induce Insulin Resistance., Macauley MS, Bubb AK, Martinez-Fleites C, Davies GJ, Vocadlo DJ, J Biol Chem. 2008 Dec 12;283(50):34687-95. Epub 2008 Oct 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18842583 18842583]
[[Category: Bubb A]]
[[Category: Bacteria]]
[[Category: Davies GJ]]
[[Category: Beta-N-acetylhexosaminidase]]
[[Category: Macauley MS]]
[[Category: Bubb, A.]]
[[Category: Martinez-Fleites C]]
[[Category: Davies, G J.]]
[[Category: Vocadlo DJ]]
[[Category: Macauley, M S.]]
[[Category: Martinez-Fleites, C.]]
[[Category: Vocadlo, D J.]]
[[Category: Glycoside hydrolase]]
[[Category: Hydrolase]]
[[Category: Inhibitor]]
[[Category: O-glcnac]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 17 12:58:40 2008''

Latest revision as of 15:34, 13 December 2023

BtGH84 structure in complex with PUGNAc

2vvs, resolution 2.24Å

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