2npx: Difference between revisions

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{{Seed}}
[[Image:2npx.png|left|200px]]


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==NADH BINDING SITE AND CATALYSIS OF NADH PEROXIDASE==
The line below this paragraph, containing "STRUCTURE_2npx", creates the "Structure Box" on the page.
<StructureSection load='2npx' size='340' side='right'caption='[[2npx]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2npx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NPX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NPX FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
{{STRUCTURE_2npx|  PDB=2npx  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2npx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2npx OCA], [https://pdbe.org/2npx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2npx RCSB], [https://www.ebi.ac.uk/pdbsum/2npx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2npx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NAPE_ENTFA NAPE_ENTFA] Peroxidase whose active site is a redox-active cysteine-sulfenic acid.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/2npx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2npx ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of the complex between cofactor NADH and the enzyme NADH peroxidase from Streptococcus faecalis 10C1 (Enterococcus faecalis) has been determined by crystal soaking, X-ray data collection, model building of NADH and refinement at 0.24-nm resolution based on the known enzyme structure [Stehle, T., Ahmed, S. A., Claiborne, A. &amp; Schulz, G. E. (1991) J. Mol. Biol. 221, 1325-1344]. Apart from NADH, the catalytic center of the enzyme contains FAD and a cysteine that shuttles between thiolate and sulfenic acid states. Unfortunately, this cysteine was irreversibly oxidized to a cysteine sulfonic acid in the established enzyme structure. Based on the geometry of the catalytic center, we discuss the stabilization of the oxidation-sensitive sulfenic acid and propose a reaction mechanism.


===NADH BINDING SITE AND CATALYSIS OF NADH PEROXIDASE===
NADH binding site and catalysis of NADH peroxidase.,Stehle T, Claiborne A, Schulz GE Eur J Biochem. 1993 Jan 15;211(1-2):221-6. PMID:8425532<ref>PMID:8425532</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2npx" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_8425532}}, adds the Publication Abstract to the page
*[[NADH peroxidase|NADH peroxidase]]
(as it appears on PubMed at http://www.pubmed.gov), where 8425532 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_8425532}}
__TOC__
 
</StructureSection>
==About this Structure==
2NPX is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NPX OCA].
 
==Reference==
<ref group="xtra">PMID:8425532</ref><references group="xtra"/>
[[Category: Enterococcus faecalis]]
[[Category: Enterococcus faecalis]]
[[Category: NADH peroxidase]]
[[Category: Large Structures]]
[[Category: Claiborne, A.]]
[[Category: Claiborne A]]
[[Category: Schulz, G E.]]
[[Category: Schulz GE]]
[[Category: Stehle, T.]]
[[Category: Stehle T]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:21:38 2009''

Latest revision as of 05:39, 5 June 2024

NADH BINDING SITE AND CATALYSIS OF NADH PEROXIDASE

2npx, resolution 2.40Å

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