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New page: left|200px<br /><applet load="2auk" size="450" color="white" frame="true" align="right" spinBox="true" caption="2auk, resolution 2.3Å" /> '''Structure of E. coli ...
 
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[[Image:2auk.gif|left|200px]]<br /><applet load="2auk" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2auk, resolution 2.3&Aring;" />
'''Structure of E. coli RNA polymerase beta' G/G' insert'''<br />


==Overview==
==Structure of E. coli RNA polymerase beta' G/G' insert==
The large beta and beta' subunits of the bacterial core RNA polymerase, (RNAP) are highly conserved throughout evolution. Nevertheless, large, sequence insertions in beta and beta' characterize specific evolutionary, lineages of bacteria. The Thermus aquaticus RNAP beta' subunit contains a, 283 residue insert between conserved regions A and B that is found in only, four bacterial species. The Escherichia coli RNAP beta' subunit contains a, 188 residue insert in the middle of conserved region G that is found in a, wide range of bacterial species. Here, we present structural studies of, these two beta' insertions. We show that the inserts comprise repeats of a, previously characterized fold, the sandwich-barrel hybrid motif (as, predicted from previous sequence analysis) and that the inserts serve, significant roles in facilitating protein/protein and/or protein/nucleic, acid interactions.
<StructureSection load='2auk' size='340' side='right'caption='[[2auk]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2auk]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AUK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2auk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2auk OCA], [https://pdbe.org/2auk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2auk RCSB], [https://www.ebi.ac.uk/pdbsum/2auk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2auk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RPOC_ECOLI RPOC_ECOLI] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_01322]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/au/2auk_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2auk ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2AUK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AUK OCA].
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure and function of lineage-specific sequence insertions in the bacterial RNA polymerase beta' subunit., Chlenov M, Masuda S, Murakami KS, Nikiforov V, Darst SA, Mustaev A, J Mol Biol. 2005 Oct 14;353(1):138-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16154587 16154587]
[[Category: DNA-directed RNA polymerase]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Chlenov, M.]]
[[Category: Chlenov M]]
[[Category: Darst, S.A.]]
[[Category: Darst SA]]
[[Category: Masuda, S.]]
[[Category: Masuda S]]
[[Category: Murakami, K.S.]]
[[Category: Murakami KS]]
[[Category: Mustaev, A.]]
[[Category: Mustaev A]]
[[Category: Nikiforov, V.]]
[[Category: Nikiforov V]]
[[Category: sandwich-barrel hybrid motif]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:22:57 2007''

Latest revision as of 09:14, 14 February 2024

Structure of E. coli RNA polymerase beta' G/G' insert

2auk, resolution 2.30Å

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