2d00: Difference between revisions

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New page: left|200px<br /><applet load="2d00" size="450" color="white" frame="true" align="right" spinBox="true" caption="2d00, resolution 2.2Å" /> '''Subunit F of V-type A...
 
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[[Image:2d00.gif|left|200px]]<br /><applet load="2d00" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2d00, resolution 2.2&Aring;" />
'''Subunit F of V-type ATPase/synthase'''<br />


==Overview==
==Subunit F of V-type ATPase/synthase==
The crystal structure of subunit F of vacuole-type ATPase/synthase, (prokaryotic V-ATPase) was determined to of 2.2 A resolution. The subunit, reveals unexpected structural similarity to the response regulator, proteins that include the Escherichia coli chemotaxis response regulator, CheY. The structure was successfully placed into the low-resolution EM, structure of the prokaryotic holo-V-ATPase at a location indicated by the, results of crosslinking experiments. The crystal structure, together with, the single-molecule analysis using fluorescence resonance energy transfer, showed that the subunit F exhibits two conformations, a 'retracted' form, in the absence and an 'extended' form in the presence of ATP. Our results, postulated that the subunit F is a regulatory subunit in the V-ATPase.
<StructureSection load='2d00' size='340' side='right'caption='[[2d00]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2d00]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D00 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d00 OCA], [https://pdbe.org/2d00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d00 RCSB], [https://www.ebi.ac.uk/pdbsum/2d00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d00 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/VATF_THET8 VATF_THET8] Produces ATP from ADP in the presence of a proton gradient across the membrane.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d0/2d00_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d00 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2D00 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2D00 OCA].
*[[ATPase 3D structures|ATPase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure of a central stalk subunit F of prokaryotic V-type ATPase/synthase from Thermus thermophilus., Makyio H, Iino R, Ikeda C, Imamura H, Tamakoshi M, Iwata M, Stock D, Bernal RA, Carpenter EP, Yoshida M, Yokoyama K, Iwata S, EMBO J. 2005 Nov 16;24(22):3974-83. Epub 2005 Nov 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16281059 16281059]
[[Category: Large Structures]]
[[Category: H(+)-transporting two-sector ATPase]]
[[Category: Single protein]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
[[Category: Bernal, R.A.]]
[[Category: Bernal RA]]
[[Category: Carpenter, E.P.]]
[[Category: Carpenter EP]]
[[Category: Iino, R.]]
[[Category: Iino R]]
[[Category: Ikeda, C.]]
[[Category: Ikeda C]]
[[Category: Imamura, H.]]
[[Category: Imamura H]]
[[Category: Iwata, M.]]
[[Category: Iwata M]]
[[Category: Iwata, S.]]
[[Category: Iwata S]]
[[Category: Makyio, H.]]
[[Category: Makyio H]]
[[Category: Stock, D.]]
[[Category: Stock D]]
[[Category: Tamakoshi, M.]]
[[Category: Tamakoshi M]]
[[Category: Yokoyama, K.]]
[[Category: Yokoyama K]]
[[Category: Yoshida, M.]]
[[Category: Yoshida M]]
[[Category: CA]]
[[Category: chey]]
[[Category: fret]]
[[Category: subunit f]]
[[Category: v-atpase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:22:22 2007''

Latest revision as of 13:45, 13 March 2024

Subunit F of V-type ATPase/synthase

2d00, resolution 2.20Å

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