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New page: left|200px<br /><applet load="2f5u" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f5u, resolution 2.10Å" /> '''Structural Character...
 
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[[Image:2f5u.gif|left|200px]]<br /><applet load="2f5u" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2f5u, resolution 2.10&Aring;" />
'''Structural Characterization of the UL25 DNA Packaging Protein from Herpes Simplex Virus Type 1'''<br />


==Overview==
==Structural Characterization of the UL25 DNA Packaging Protein from Herpes Simplex Virus Type 1==
Herpesviruses replicate their double stranded DNA genomes as, high-molecular-weight concatemers which are subsequently cleaved into, unit-length genomes by a complex mechanism that is tightly coupled to DNA, insertion into a preformed capsid structure, the procapsid. The herpes, simplex virus type 1 UL25 protein is incorporated into the capsid during, DNA packaging, and previous studies of a null mutant have demonstrated, that its function is essential at the late stages of the head-filling, process, either to allow packaging to proceed to completion or for, retention of the viral genome within the capsid. We have expressed and, purified an N-terminally truncated form of the 580-residue UL25 protein, and have determined the crystallographic structure of the region, corresponding to amino acids 134 to 580 at 2.1-Angstroms resolution. This, structure, the first for any herpesvirus protein involved in processing, and packaging of viral DNA, reveals a novel fold, a distinctive, electrostatic distribution, and a unique "flexible" architecture in which, numerous flexible loops emanate from a stable core. Evolutionary trace, analysis of UL25 and its homologues in other herpesviruses was used to, locate potentially important amino acids on the surface of the protein, leading to the identification of four putative docking regions for protein, partners.
<StructureSection load='2f5u' size='340' side='right'caption='[[2f5u]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2f5u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_alphaherpesvirus_1 Human alphaherpesvirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F5U FirstGlance]. <br>
2F5U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_4 Human herpesvirus 4]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2F5U OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5u OCA], [https://pdbe.org/2f5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f5u RCSB], [https://www.ebi.ac.uk/pdbsum/2f5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f5u ProSAT]</span></td></tr>
==Reference==
</table>
Structural characterization of the UL25 DNA-packaging protein from herpes simplex virus type 1., Bowman BR, Welschhans RL, Jayaram H, Stow ND, Preston VG, Quiocho FA, J Virol. 2006 Mar;80(5):2309-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16474137 16474137]
== Function ==
[[Category: Human herpesvirus 4]]
[https://www.uniprot.org/uniprot/CVC2_HHV11 CVC2_HHV11] Capsid vertex-specific component that plays a role during viral DNA encapsidation, assuring correct genome cleavage and presumably stabilizing capsids that contain full-length viral genomes. Participates in the interaction between the capsid and the tegument through interaction with the large tegument protein/LTP. May mediate the capsid docking to the nuclear pore allowing entry of the viral genome into the host nucleus through binding to host nucleoporins NUP214.[HAMAP-Rule:MF_04025]<ref>PMID:18448531</ref> <ref>PMID:18945788</ref>
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Bowman, B.R.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: capsid protein]]
Check<jmol>
[[Category: dna packaging]]
  <jmolCheckbox>
[[Category: head completion]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f5/2f5u_consurf.spt"</scriptWhenChecked>
[[Category: hsv-1]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: ul25]]
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:22:51 2007''
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f5u ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Human alphaherpesvirus 1]]
[[Category: Large Structures]]
[[Category: Bowman BR]]

Latest revision as of 09:21, 14 February 2024

Structural Characterization of the UL25 DNA Packaging Protein from Herpes Simplex Virus Type 1

2f5u, resolution 2.10Å

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