3sfp: Difference between revisions
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[[ | ==Crystal Structure of the Mono-Zinc-boundform of New Delhi Metallo-beta-Lactamase-1 from Klebsiella pneumoniae== | ||
<StructureSection load='3sfp' size='340' side='right' caption='[[3sfp]], [[Resolution|resolution]] 2.27Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3sfp]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SFP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SFP FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rkj|3rkj]], [[3rkk|3rkk]], [[3sbl|3sbl]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">blaNDM-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sfp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sfp RCSB], [http://www.ebi.ac.uk/pdbsum/3sfp PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The New Delhi Metallo-beta-lactamase (NDM-1) gene makes multiple pathogenic microorganisms resistant to all known beta-lactam antibiotics. The rapid emergence of NDM-1 has been linked to mobile plasmids that move between different strains resulting in world-wide dissemination. Biochemical studies revealed that NDM-1 is capable of efficiently hydrolyzing a wide range of beta-lactams, including many carbapenems considered as "last resort" antibiotics. The crystal structures of metal-free apo- and monozinc forms of NDM-1 presented here revealed an enlarged and flexible active site of class B1 metallo-beta-lactamase. This site is capable of accommodating many beta-lactam substrates by having many of the catalytic residues on flexible loops, which explains the observed extended spectrum activity of this zinc dependent beta-lactamase. Indeed, five loops contribute "keg" residues in the active site including side chains involved in metal binding. Loop 1 in particular, shows conformational flexibility, apparently related to the acceptance and positioning of substrates for cleavage by a zinc-activated water molecule. | |||
Structure of apo- and monometalated forms of NDM-1--a highly potent carbapenem-hydrolyzing metallo-beta-lactamase.,Kim Y, Tesar C, Mire J, Jedrzejczak R, Binkowski A, Babnigg G, Sacchettini J, Joachimiak A PLoS One. 2011;6(9):e24621. Epub 2011 Sep 8. PMID:21931780<ref>PMID:21931780</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Beta-lactamase|Beta-lactamase]] | *[[Beta-lactamase|Beta-lactamase]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: Beta-lactamase]] | [[Category: Beta-lactamase]] | ||
[[Category: Klebsiella pneumoniae]] | [[Category: Klebsiella pneumoniae]] | ||
[[Category: Babnigg, J | [[Category: Babnigg, J]] | ||
[[Category: Binkowski, T A | [[Category: Binkowski, T A]] | ||
[[Category: Jedrzejczak, R | [[Category: Jedrzejczak, R]] | ||
[[Category: Joachimiak, A | [[Category: Joachimiak, A]] | ||
[[Category: Kim, Y | [[Category: Kim, Y]] | ||
[[Category: | [[Category: Structural genomic]] | ||
[[Category: MTBI, Structures of Mtb Proteins Conferring Susceptibility to Known Mtb Inhibitors | [[Category: MTBI, Structures of Mtb Proteins Conferring Susceptibility to Known Mtb Inhibitors]] | ||
[[Category: Tesar, C | [[Category: Tesar, C]] | ||
[[Category: Alpha-beta-beta-alpha sandwich]] | [[Category: Alpha-beta-beta-alpha sandwich]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Mcsg]] | [[Category: Mcsg]] | ||
[[Category: Mtbi]] | [[Category: Mtbi]] | ||
[[Category: Periplasmic]] | [[Category: Periplasmic]] | ||
[[Category: Psi-biology]] | [[Category: Psi-biology]] | ||
[[Category: Structures of mtb proteins conferring susceptibility to known mtb inhibitor]] | [[Category: Structures of mtb proteins conferring susceptibility to known mtb inhibitor]] | ||
Revision as of 12:40, 9 December 2014
Crystal Structure of the Mono-Zinc-boundform of New Delhi Metallo-beta-Lactamase-1 from Klebsiella pneumoniae
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Proteopedia Page Contributors and Editors (what is this?)
Categories:
- Beta-lactamase
- Klebsiella pneumoniae
- Babnigg, J
- Binkowski, T A
- Jedrzejczak, R
- Joachimiak, A
- Kim, Y
- Structural genomic
- MTBI, Structures of Mtb Proteins Conferring Susceptibility to Known Mtb Inhibitors
- Tesar, C
- Alpha-beta-beta-alpha sandwich
- Hydrolase
- Mcsg
- Mtbi
- Periplasmic
- Psi-biology
- Structures of mtb proteins conferring susceptibility to known mtb inhibitor
