4dm0: Difference between revisions
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===TN5 transposase: 20MER OUTSIDE END 2 MN complex=== | ===TN5 transposase: 20MER OUTSIDE END 2 MN complex=== | ||
{{ABSTRACT_PUBMED_11896402}} | {{ABSTRACT_PUBMED_11896402}} | ||
==Function== | |||
[[http://www.uniprot.org/uniprot/TN5P_ECOLX TN5P_ECOLX]] Mediates transposition of transposon Tn5 by a 'cut and paste' mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After nicking of the first DNA strand, excision of the transposon proceeds through a series of intermediates. The transposase then mediates the insertion of the transposon at a new site by strand transfer. The activity of the wild-type transposase is very low, and is further inhibited by dimerization with the transposase inhibitor (inh).<ref>PMID:6260374</ref><ref>PMID:6291786</ref><ref>PMID:6303899</ref><ref>PMID:1310499</ref><ref>PMID:8226636</ref><ref>PMID:8871560</ref><ref>PMID:11877443</ref><ref>PMID:12367522</ref> | |||
==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
<ref group="xtra">PMID:011896402</ref><references group="xtra"/> | <ref group="xtra">PMID:011896402</ref><references group="xtra"/><references/> | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Goryshin, I Y.]] | [[Category: Goryshin, I Y.]] | ||
