3ngg: Difference between revisions
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==X-ray Structure of Omwaprin== | |||
=== | <StructureSection load='3ngg' size='340' side='right' caption='[[3ngg]], [[Resolution|resolution]] 1.33Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3ngg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NGG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NGG FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ngg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ngg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ngg RCSB], [http://www.ebi.ac.uk/pdbsum/3ngg PDBsum]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ng/3ngg_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The 50-residue snake venom protein L-omwaprin and its enantiomer D-omwaprin were prepared by total chemical synthesis. Radial diffusion assays were performed against Bacillus megaterium and Bacillus anthracis; both L-omwaprin and D-omwaprin showed anti-bacterial activity against B. megaterium. The native protein enantiomer, made of L- amino acids, failed to crystallize readily. However, when a racemic mixture containing equal amounts of L-omwaprin and D-omwaprin was used, diffraction quality crystals were obtained. The racemic protein sample crystallized in the centrosymmetric space group P2(1)/c and its structure was determined at atomic resolution (1.33 A) by a combination of Patterson and direct methods based on the strong scattering from the sulfur atoms in the eight cysteine residues per protein. Racemic crystallography once again proved to be a valuable method for obtaining crystals of recalcitrant proteins, and for determining high resolution X-ray structures by direct methods. | |||
Determination of the X-ray structure of the snake venom protein omwaprin by total chemical synthesis and racemic protein crystallography.,Banigan JR, Mandal K, Sawaya MR, Thammavongsa V, Hendrickx AP, Schneewind O, Yeates TO, H Kent SB Protein Sci. 2010 Jul 28. PMID:20669184<ref>PMID:20669184</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
[[Category: Banigan, J R | == References == | ||
[[Category: Hendrickx, A P.A | <references/> | ||
[[Category: Kent, S B.H | __TOC__ | ||
[[Category: Mandal, K | </StructureSection> | ||
[[Category: Sawaya, M R | [[Category: Banigan, J R]] | ||
[[Category: Schneewind, O | [[Category: Hendrickx, A P.A]] | ||
[[Category: Thammavongsa, V | [[Category: Kent, S B.H]] | ||
[[Category: Yeates, T O | [[Category: Mandal, K]] | ||
[[Category: Sawaya, M R]] | |||
[[Category: Schneewind, O]] | |||
[[Category: Thammavongsa, V]] | |||
[[Category: Yeates, T O]] | |||
[[Category: Antibiotic]] | [[Category: Antibiotic]] | ||
[[Category: Direct method]] | [[Category: Direct method]] | ||
[[Category: Racemic protein crystallography]] | [[Category: Racemic protein crystallography]] | ||
[[Category: Venom protein]] | [[Category: Venom protein]] | ||
