3ksk

Crystal Structure of single chain PvuII

spinqualitylabelsall models 3ksk, resolution 2.35Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3ksk is a 2 chain structure with sequence from Proteus vulgaris. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MPD, SO4, TRS Related: 1pvu, 1pvi Gene: pvuII, pvuIIR (Proteus vulgaris) Activity: Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 Resources: FirstGlance, OCA, RCSB, PDBsum Function [T2P2_PROVU] Recognizes the double-stranded sequence CAGCTG and cleaves after G-3. Publication Abstract from PubMed The restriction endonuclease PvuII from Proteus vulgaris has been converted from its wild-type homodimeric form into the enzymatically active single-chain variant scPvuII by tandemly joining the two subunits through the peptide linker Gly-Ser-Gly-Gly. scPvuII, which is suitable for the development of programmed restriction endonucleases for highly specific DNA cleavage, was purified and crystallized. The crystals diffract to a resolution of 2.35 A and belong to space group P4(2), with unit-cell parameters a = b = 101.92, c = 100.28 A and two molecules per asymmetric unit. Phasing was successfully performed by molecular replacement. Purification, crystallization, X-ray diffraction analysis and phasing of an engineered single-chain PvuII restriction endonuclease.,Meramveliotaki C, Kotsifaki D, Androulaki M, Hountas A, Eliopoulos E, Kokkinidis M Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Oct 1;63(Pt, 10):836-8. Epub 2007 Sep 19. PMID:17909283 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Endonuclease

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