4na1: Difference between revisions

Revision as of 08:48, 4 August 2016

Crystal Structure of the second ketosynthase from the bacillaene polyketide synthase

Structural highlights

4na1 is a 2 chain structure with sequence from Bacsu. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	SO4
Related:	2qo3, 2hg4, 4na2, 4na3
Gene:	pksJ, pksK, BSU17180 (BACSU)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PKSJ_BACSU] Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism.^[1] ^[2]

Publication Abstract from PubMed

The recently discovered trans-acyltransferase modular polyketide synthases catalyze the biosynthesis of a wide range of bioactive natural products in bacteria. Here we report the structure of the second ketosynthase from the bacillaene trans-acyltransferase polyketide synthase. This 1.95 A resolution structure provides the highest resolution view available of a modular polyketide synthase ketosynthase and reveals a flanking subdomain that is homologous to an ordered linker in cis-acyltransferase modular polyketide synthases. The structure of the cysteine-to-serine mutant of the ketosynthase acylated by its natural substrate provides high-resolution details of how a native polyketide intermediate is bound and helps explain the basis of ketosynthase substrate specificity. The substrate range of the ketosynthase was further investigated by mass spectrometry.

A Close Look at a Ketosynthase from a Trans-Acyltransferase Modular Polyketide Synthase.,Gay DC, Gay G, Axelrod AJ, Jenner M, Kohlhaas C, Kampa A, Oldham NJ, Piel J, Keatinge-Clay AT Structure. 2014 Feb 4. pii: S0969-2126(14)00008-2. doi:, 10.1016/j.str.2013.12.016. PMID:24508341^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dorrestein PC, Blackhall J, Straight PD, Fischbach MA, Garneau-Tsodikova S, Edwards DJ, McLaughlin S, Lin M, Gerwick WH, Kolter R, Walsh CT, Kelleher NL. Activity screening of carrier domains within nonribosomal peptide synthetases using complex substrate mixtures and large molecule mass spectrometry. Biochemistry. 2006 Feb 14;45(6):1537-46. PMID:16460000 doi:https://dx.doi.org/10.1021/bi052333k
↑ Butcher RA, Schroeder FC, Fischbach MA, Straight PD, Kolter R, Walsh CT, Clardy J. The identification of bacillaene, the product of the PksX megacomplex in Bacillus subtilis. Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1506-9. Epub 2007 Jan 18. PMID:17234808 doi:https://dx.doi.org/10.1073/pnas.0610503104
↑ Gay DC, Gay G, Axelrod AJ, Jenner M, Kohlhaas C, Kampa A, Oldham NJ, Piel J, Keatinge-Clay AT. A Close Look at a Ketosynthase from a Trans-Acyltransferase Modular Polyketide Synthase. Structure. 2014 Feb 4. pii: S0969-2126(14)00008-2. doi:, 10.1016/j.str.2013.12.016. PMID:24508341 doi:https://dx.doi.org/10.1016/j.str.2013.12.016

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Dorrestein PC, Blackhall J, Straight PD, Fischbach MA, Garneau-Tsodikova S, Edwards DJ, McLaughlin S, Lin M, Gerwick WH, Kolter R, Walsh CT, Kelleher NL. Activity screening of carrier domains within nonribosomal peptide synthetases using complex substrate mixtures and large molecule mass spectrometry. Biochemistry. 2006 Feb 14;45(6):1537-46. PMID:16460000 doi:https://dx.doi.org/10.1021/bi052333k

[2] Butcher RA, Schroeder FC, Fischbach MA, Straight PD, Kolter R, Walsh CT, Clardy J. The identification of bacillaene, the product of the PksX megacomplex in Bacillus subtilis. Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1506-9. Epub 2007 Jan 18. PMID:17234808 doi:https://dx.doi.org/10.1073/pnas.0610503104

[3] Gay DC, Gay G, Axelrod AJ, Jenner M, Kohlhaas C, Kampa A, Oldham NJ, Piel J, Keatinge-Clay AT. A Close Look at a Ketosynthase from a Trans-Acyltransferase Modular Polyketide Synthase. Structure. 2014 Feb 4. pii: S0969-2126(14)00008-2. doi:, 10.1016/j.str.2013.12.016. PMID:24508341 doi:https://dx.doi.org/10.1016/j.str.2013.12.016

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@@ Line 1: / Line 1: @@
 ==Crystal Structure of the second ketosynthase from the bacillaene polyketide synthase==
 <StructureSection load='4na1' size='340' side='right' caption='[[4na1]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2qo3|2qo3]], [[2hg4|2hg4]], [[4na2|4na2]], [[4na3|4na3]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pksJ, pksK, BSU17180 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4na1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4na1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4na1 RCSB], [http://www.ebi.ac.uk/pdbsum/4na1 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4na1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4na1 OCA], [http://pdbe.org/4na1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4na1 RCSB], [http://www.ebi.ac.uk/pdbsum/4na1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4na1 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4na1" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4na1: Difference between revisions

Revision as of 08:48, 4 August 2016

Crystal Structure of the second ketosynthase from the bacillaene polyketide synthase

Structural highlights

Function

Publication Abstract from PubMed

References

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