1jch

Crystal Structure of Colicin E3 in Complex with its Immunity Protein

spinqualitylabelsall models 1jch, resolution 3.02Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1jch is a 4 chain structure with sequence from Ecow3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CIT, GOL Related: 3eip, 1e44 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [CEA3_ECOLX] Inactivates ribosomes by hydrolyzing 16S RNA in 30S ribosomes at a specific site. Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria. [IMM3_ECOLX] This protein inhibits the 16S RNA hydrolyzing activity of colicin E3 by binding with high affinity to the C-terminal catalytic domain of E3. This protein is able to protect a cell, which harbors the plasmid ColE3 against colicin E3. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Colicins kill E. coli by a process that involves binding to a surface receptor, entering the cell, and, finally, intoxicating it. The lethal action of colicin E3 is a specific cleavage in the ribosomal decoding A site. The crystal structure of colicin E3, reported here in a binary complex with its immunity protein (IP), reveals a Y-shaped molecule with the receptor binding domain forming a 100 A long stalk and the two globular heads of the translocation domain (T) and the catalytic domain (C) comprising the two arms. Active site residues are D510, H513, E517, and R545. IP is buried between T and C. Rather than blocking the active site, IP prevents access of the active site to the ribosome. Crystal structure of colicin E3: implications for cell entry and ribosome inactivation.,Soelaiman S, Jakes K, Wu N, Li C, Shoham M Mol Cell. 2001 Nov;8(5):1053-62. PMID:11741540 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Colicin Colicin Immunity Protein Im3

Proteopedia Page Contributors and Editors (what is this?)

OCA