Transposase

spinqualitylabelsall models Structure of Tn5 transposase complex with DNA and glycerol (PDB code 3ecp). Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Transposases bind to the ends of a transposon and catalyze its movement to another part of the genome by a cut-and-paste mechanism. Mu transposase (MuA) is essential for integration, replication-transposition and excision of bacteriophage Mu DNA into multiple sites of bacterial genome. Mu transposition occurs within the transposome which is a protein-DNA complex which includes 4 subunits of MuA. Hermes transposase is a fly protein. Dra2 transposase is a Deinococcus radiodurans protein Tn5 transposase active site contains the DDE motif which catalyzes the movement of the transposon. Tn7 transposase is bacterial and highly specific. Mos1 transposase mediates the movement of Mos1 transposon Tc3 transposase mediates the movement of Tc3 transposon in C. elegans Sleeping beauty transposase synthetic transposon developed as a non-viral vector for gene therapy Structural highlights Transposase contains a DDE motif in its active site enabling it to coordinate the binding of divalent metal ions. The metal ions affect transposase reactivity. The DDE motif is conserved in transposases and retroviral integrases and is essential for their activity.

3D structures of transposase

Updated on 21-September-2016

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• Mu transposase
  • 1tns, 1tnt – MuMuA DNA-binding domain – enterobacteria phage Mu - NMR
    
  • 1bcm, 1bco – MuMuA core domain
    
  • 2ezh, 2ezi – MuMuA Iγ subdomain - NMR
    
  • 2ezk – MuMuA Iβ subdomain - NMR
    
  • 4fcy – MuMuA + DNA
    
• Hermes transposase
  • 2bw3 – fTra (mutant) - fly
    
  • 4d1q – fTra (mutant) + DNA
    
• Dra2 transposase
  • 2xm3 – Dra2 + Dra2 transposase binding element + DNA – Deinococcus radiodurans
    
  • 2xma, 2xqc – Dra2 + DNA
    
  • 2xo6 – Dra2 (mutant) + DNA
    
• Tn5 transposase
  • 1muh, 1mus, 1mm8, 3ecp, 4dm0 – EcTn5 (mutant) + DNA – Escherichia coli
    
• Tn7 transposase
  • 5d17 – EcTn7 C terminal
    
  • 5d16 – EcTn7 C terminal (mutant)
    
• Mos1 transposase
  • 2f7t – DmMos1 catalytic domain (mutant) – Drosophila mauritiana
    
  • 4u7b, 5hoo – DmMos1 + DNA
    
  • 3hos, 3hot, 4r79 – DmMos1 (mutant) + DNA
    
  • 4mda, 4mdb – DmMos1 catalytic domain (mutant) + drug
    
• Tc3 transposase
  • 1tc3, 1u78 – Tc3 DNA-binding domain (mutant) + DNA – Caenorhabditis elegans
    
• Orfa transposase (Is608)
  • 2a6m – HpOrfa – Helicobacter pylori
    
  • 2vhg, 2a6o, 2vic, 2vih, 2vju, 2vjv – HpOrfa + DNA
    
• Sleeping beauty transposase
  • 5cr4 – SB catalytic domain – synthetic
    

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Michal Harel, Alexander Berchansky