6iya: Difference between revisions
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==Structure of the DNA binding domain of antitoxin CopASO== | ==Structure of the DNA binding domain of antitoxin CopASO== | ||
<StructureSection load='6iya' size='340' side='right' caption='[[6iya]], [[Resolution|resolution]] 3.00Å' scene=''> | <StructureSection load='6iya' size='340' side='right'caption='[[6iya]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6iya]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IYA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IYA FirstGlance]. <br> | <table><tr><td colspan='2'>[[6iya]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Sheon Sheon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IYA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IYA FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6iya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iya OCA], [http://pdbe.org/6iya PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iya RCSB], [http://www.ebi.ac.uk/pdbsum/6iya PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iya ProSAT]</span></td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SO_1445 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=211586 SHEON])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6iya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iya OCA], [http://pdbe.org/6iya PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iya RCSB], [http://www.ebi.ac.uk/pdbsum/6iya PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iya ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Toxin-antitoxin (TA) systems play critical roles in the environment adaptation of bacteria. Allosteric coupling between the N-terminal DNA-binding domain and the C-terminal toxin-binding domain of antitoxins contributes to conditional cooperativity in the functioning of type II TA. Herein, using circular dichroism (CD), nuclear magnetic resonance (NMR), X-ray crystallography, and size exclusion chromatography (SEC), the structure and DNA binding of CopASO, a newly identified type II antitoxin in Shewanella oneidensis, were investigated. Our data show that CopASO is a typical RHH antitoxin with an ordered N-terminal domain and a disordered C-terminal domain, and furthermore indicate that the C-terminal domain facilitates DNA binding of the N-terminal domain, which in turn induces the C-terminal domain to fold and associate. | |||
Structure and allosteric coupling of type antitoxin CopASO.,Zhao R, Li Q, Zhang J, Li F, Yao J, Zhang J, Liu L, Wang X, Zhang X Biochem Biophys Res Commun. 2019 Jul 5;514(4):1122-1127. doi:, 10.1016/j.bbrc.2019.05.049. Epub 2019 May 14. PMID:31101334<ref>PMID:31101334</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6iya" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Sheon]] | |||
[[Category: Li, F]] | [[Category: Li, F]] | ||
[[Category: Liu, L]] | [[Category: Liu, L]] | ||
Revision as of 06:16, 7 August 2019
Structure of the DNA binding domain of antitoxin CopASO
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