2f7k

Crystal Structure of Human Pyridoxal Kinase

spinqualitylabelsall models 2f7k, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2f7k is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Activity: Pyridoxal kinase, with EC number 2.7.1.35 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [PDXK_HUMAN] Required for synthesis of pyridoxal-5-phosphate from vitamin B6. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Pyridoxal kinase, a member of the ribokinase superfamily, catalyzes the ATP-dependent phosphorylation reaction of vitamin B6 and is an essential enzyme in the formation of pyridoxal-5'-phosphate, a key cofactor for over 100 enzymes. Pyridoxal kinase is thus regarded as a potential target for pharmacological agents. In this paper, we report the 2.8 angstroms crystal structure of human pyridoxal kinase (HPLK) expressed in Escherichia coli. The diffraction data revealed unexpected merohedral perfect twinning along the crystallographic c axis. Taking perfect twinning into account, the structure in dimeric form was well refined according to the CNS program. Structure comparison reveals that the key 12-residue peptide over the active site in HPLK is a beta-strand/loop/beta-strand flap, while the corresponding peptide in sheep brain enzyme adopts a loop conformation. Moreover, HPLK possesses a more hydrophobic ATP-binding pocket. This structure will facilitate further biochemical studies and structure-based design of drugs related to pyridoxal kinase. Crystal structure of human pyridoxal kinase.,Cao P, Gong Y, Tang L, Leung YC, Jiang T J Struct Biol. 2006 Jun;154(3):327-32. Epub 2006 Mar 20. PMID:16600635 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Pyridoxal kinase

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