Vinculin

spinqualitylabelsall models Chicken full-length metavinculin, 1st6 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Vinculins (VCLs) are involved in adhesion by linking integrin molecules to the actin cytoskeleton. Its head domain (Vd1) can bind to talin or to alpha-actinin at their respective VCL Binding Sites (VBS). The protein raver1 RNA Recognition Motif (RRM) forms a complex with VCL or m-VCL. Metavinculin (m-VCL) is a splice version of VCL containing an extra ca. 70 amino acids in the C-terminal domain. Relevance Loss of VCL could be used as a prognostic factor for colorectal cancer se it promotes metastasis. Disease Mutation in m-VCL can yield cardiomyopathic phenotype. Structural highlights Vinculin Autoinhibition is achieved through a high affinity intramolecular interaction between tail (orange) and head (aqua) domains (1st6). Energetically, I997 is key to maintaining this autoinhibition.

3D Structures of Vinculin

Updated on 23-June-2022

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• Vinculin; Domains - head 140-327; tail 878-1066
  • talin, alpha-actinin – hVCL - human
    
  • 1st6 – hVCL head domain + raver1 RRM
    
  • 2ibf, 2hsq, 2gww - hVCL head domain + SfVCL binding sites from Shigella flexneri
    
  • 1ydi - hVCL head domain +hActinin VBS
    
  • 1syq, 1rkc, 1rke – hVCL head domain +hTalin VBS
    
  • 1qkr – hVCL tail domain
    
  • 3h2v - hVCL tail domain + raver1 RRM
    
  • 4pr9 - hVCL tail domain + lipid
    
  • 3jbi - hVCL tail domain + actin – Cryo-EM
    
  • 6nr7 - cVCL – chicken
    
  • 1t01 - cVCL head domain +mTalin VBS
    
  • 3zdl – cVCL head domain + amyloid β precursor protein N terminal
    
• Metavinculin; Domains - head 1-256; tail 959-1134
  • 7ktt, 7ktu, 7ktv, 7ktw – hVCL – Cryo-EM
    
  • 6upw – hVCL + actin + ADP – Cryo-EM
    
  • 6fuy - hVCL
    
  • 3rf3 - hVCL head domain + invasin IPAA
    
  • 3s90 - hVCL head domain + mTalin-1 peptide
    
  • 4dj9 - hVCL head domain + hTalin-1 peptide
    
  • 6fq4 - hVCL head domain + TARP peptide
    
  • 3tj5 - hVCL head domain + Sca-family protein peptide
    
  • 3tj6 - hVCL head domain + protein Ps 120 peptide
    
  • 4ehp, 5y04 - hVCL head domain + catenin α-1 residues 277-382
    
  • 3myi – hVCL tail domain
    
  • 5l0f, 5l0i, 5l0j - hVCL tail domain (mutant)
    
  • 5l0c, 5l0d - hVCL tail domain + lipid
    
  • 5l0g, 5l0h - hVCL tail domain (mutant) + lipid
    
  • 3jbk – hVCL tail domain + actin – Cryo-EM
    
  • 6upw - mVCL head domain + catenin α-1
    
  • 3vf0 – hVCL tail domain + ribonucleoprotein PTB-binding
    
  • 1st6 – cVCL
    
  • 2gdc – cVCL head domain +SfInvasin C-terminal
    
  • 1xwj - cVCL head domain +cTalin VBS3
    
  • 1zvz, 1zw2, 1zw3, 1u6h - cVCL head domain +cTalin rod
    
  • 6fq4 – cVCL head domain + TARP-VBS1
    
  • 4e17, 4e18 - cVCL head domain + catenin α-1 VCL-binding domain

References

• Created with the participation of Susan Craig.

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Alexander Berchansky, Jaime Prilusky, Michal Harel