5tbz

E. Coli RNA Polymerase complexed with NusG

spinqualitylabelsall models 5tbz, resolution 7.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5tbz is a 10 chain structure with sequence from Escherichia coli O157:H7 and Escherichia coli S88. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 7Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RPOC_ECOLI DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_01322] Publication Abstract from PubMed NusG is an essential transcription factor that plays multiple key regulatory roles in transcription elongation, termination and coupling translation and transcription. The core role of NusG is to enhance transcription elongation and RNA polymerase processivity. Here, we present the structure of Escherichia coli RNA polymerase complexed with NusG. The structure shows that the NusG N-terminal domain (NGN) binds at the central cleft of RNA polymerase surrounded by the beta' clamp helices, the beta protrusion, and the beta lobe domains to close the promoter DNA binding channel and constrain the beta' clamp domain, but with an orientation that is different from the one observed in the archaeal beta' clamp-Spt4/5 complex. The structure also allows us to construct a reliable model of the complete NusG-associated transcription elongation complex, suggesting that the NGN domain binds at the upstream fork junction of the transcription elongation complex, similar to sigma2 in the transcription initiation complex, to stabilize the junction, and therefore enhances transcription processivity. Structural insights into NusG regulating transcription elongation.,Liu B, Steitz TA Nucleic Acids Res. 2016 Nov 29. pii: gkw1159. PMID:27899640 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also RNA polymerase 3D structures

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