1tq2: Difference between revisions

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[[Image:1tq2.jpg|left|200px]]
{{Seed}}
[[Image:1tq2.png|left|200px]]


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{{STRUCTURE_1tq2|  PDB=1tq2  |  SCENE=  }}  
{{STRUCTURE_1tq2|  PDB=1tq2  |  SCENE=  }}  


'''Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases'''
===Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases===




==Overview==
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Interferon-inducible p47 GTPases are critical mediators of cell-autonomous resistance against several intracellular pathogens. Here we present the first crystal structure of a member of this novel GTPase family, IIGP1, in its nucleotide-free, GDP-, and GppNHp-bound form. The structure shows a Ras-like G domain between an N-terminal three-helix bundle and a complex system of C-terminal helices and loops. Sequence comparison and secondary structure prediction suggest the IIGP1 structure to be a valid model for the p47 GTPase family. The IIGP1 crystals contain a noncrystallographic dimer. We show that the dimer is required for cooperative GTP hydrolysis and GTP-dependent oligomerization of IIGP1. We also present the GDP- and GppNHp-bound monomeric structures of two dimer interface mutants. Our structures direct approaches to the analysis of the catalytic mechanism of IIGP1 and provide a coherent basis for structure-function studies aimed at elucidating the mechanistic basis of pathogen resistance caused by these enigmatic GTPases.
The line below this paragraph, {{ABSTRACT_PUBMED_15350217}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_15350217}}


==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structure of IIGP1: a paradigm for interferon-inducible p47 resistance GTPases., Ghosh A, Uthaiah R, Howard J, Herrmann C, Wolf E, Mol Cell. 2004 Sep 10;15(5):727-39. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15350217 15350217]
Crystal structure of IIGP1: a paradigm for interferon-inducible p47 resistance GTPases., Ghosh A, Uthaiah R, Howard J, Herrmann C, Wolf E, Mol Cell. 2004 Sep 10;15(5):727-39. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15350217 15350217]
IIGP1, an interferon-gamma-inducible 47-kDa GTPase of the mouse, showing cooperative enzymatic activity and GTP-dependent multimerization., Uthaiah RC, Praefcke GJ, Howard JC, Herrmann C, J Biol Chem. 2003 Aug 1;278(31):29336-43. Epub 2003 May 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12732635 12732635]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Immunology]]
[[Category: Immunology]]
[[Category: Interferon gamma]]
[[Category: Interferon gamma]]
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