23vj: Difference between revisions
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==Crystal structure of the MafR protein from Enterococcus faecalis== | |||
<StructureSection load='23vj' size='340' side='right'caption='[[23vj]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[23vj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=23VJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=23VJ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=23vj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=23vj OCA], [https://pdbe.org/23vj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=23vj RCSB], [https://www.ebi.ac.uk/pdbsum/23vj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=23vj ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Phosphotransferase system regulatory domain-containing virulence regulators (PCVRs), including Enterococcus faecalis MafR and Streptococcus pneumoniae Mga-like protein (MgaSpn), are transcription factors in pathogenic bacteria that control the transcription of virulence genes by binding dsDNA in promoter regions. Although PCVRs play critical roles in bacterial pathogenesis, the structural and molecular basis by which PCVRs recognize dsDNA for transcriptional regulation has remained unclear. Here, we present the unique structure of MafR and demonstrate the critical role of its two N-terminal helix-turn-helix (HTH) domains in dsDNA binding. MafR adopts a five-domain architecture with an open-ring conformation and dimerizes into a table-like structure, in which the C-terminal domain mediates dimerization in the middle of the dimer structure, and the two N-terminal HTH domains project from one face of the dimer. Our extensive modeling, biochemical, and mutational analyses of the MgaSpn-dsDNA interaction reveal that the tandem HTH domains are required for dsDNA binding by inserting their recognition helices into the major groove of dsDNA. Our findings highlight a unique mode of DNA recognition by PCVRs, in which two HTH domains are simultaneously employed for DNA binding, rather than the single HTH domain typically used by conventional HTH-containing transcriptional regulators. | |||
Unique structural features and DNA recognition mechanisms of the PRD-containing virulence regulators MafR and MgaSpn.,Park MA, Ahn SY, Cho SY, Yoon SI Biochem Biophys Res Commun. 2026 May 21;814:153625. doi: , 10.1016/j.bbrc.2026.153625. Epub 2026 Mar 17. PMID:41865467<ref>PMID:41865467</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 23vj" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Enterococcus faecalis]] | |||
[[Category: Large Structures]] | |||
[[Category: Ahn SY]] | |||
[[Category: Park MA]] | |||
[[Category: Yoon SI]] | |||
Latest revision as of 08:04, 29 April 2026
Crystal structure of the MafR protein from Enterococcus faecalis
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