2b6b: Difference between revisions

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[[Image:2b6b.gif|left|200px]]<br />

[[Image:2b6b.gif|left|200px]]<br /><applet load="2b6b" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="2b6b" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="2b6b" />

'''Cryo EM structure of Dengue complexed with CRD of DC-SIGN'''<br />

==Overview==

Dengue virus (DENV) is a significant human pathogen that causes millions, of infections and results in about 24,000 deaths each year. Dendritic, cell-specific ICAM3 grabbing nonintegrin (DC-SIGN), abundant in immature, dendritic cells, was previously reported as being an ancillary receptor, interacting with the surface of DENV. The structure of DENV in complex, with the carbohydrate recognition domain (CRD) of DC-SIGN was determined, by cryo-electron microscopy at 25 A resolution. One CRD monomer was found, to bind to two glycosylation sites at Asn67 of two neighboring, glycoproteins in each icosahedral asymmetric unit, leaving the third Asn67, residue vacant. The vacancy at the third Asn67 site is a result of the, nonequivalence of the glycoprotein environments, leaving space for the, primary receptor binding to domain III of E. The use of carbohydrate, moieties for receptor binding sites suggests a mechanism for avoiding, immune surveillance.

Dengue virus (DENV) is a significant human pathogen that causes millions of infections and results in about 24,000 deaths each year. Dendritic cell-specific ICAM3 grabbing nonintegrin (DC-SIGN), abundant in immature dendritic cells, was previously reported as being an ancillary receptor interacting with the surface of DENV. The structure of DENV in complex with the carbohydrate recognition domain (CRD) of DC-SIGN was determined by cryo-electron microscopy at 25 A resolution. One CRD monomer was found to bind to two glycosylation sites at Asn67 of two neighboring glycoproteins in each icosahedral asymmetric unit, leaving the third Asn67 residue vacant. The vacancy at the third Asn67 site is a result of the nonequivalence of the glycoprotein environments, leaving space for the primary receptor binding to domain III of E. The use of carbohydrate moieties for receptor binding sites suggests a mechanism for avoiding immune surveillance.

==About this Structure==

2B6B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Dengue_virus Dengue virus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2B6B OCA].

2B6B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Dengue_virus Dengue virus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B6B OCA].

==Reference==

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[[Category: Homo sapiens]]

[[Category: Protein complex]]

[[Category: Bator-Kelly, C.M.]]

[[Category: Bator-Kelly, C M.]]

[[Category: Battisti, A.J.]]

[[Category: Battisti, A J.]]

[[Category: Chipman, P.R.]]

[[Category: Chipman, P R.]]

[[Category: Gregorio, G.]]

[[Category: Hendrickson, W.A.]]

[[Category: Hendrickson, W A.]]

[[Category: Kuhn, R.J.]]

[[Category: Kuhn, R J.]]

[[Category: Pokidysheva, E.]]

[[Category: Rossmann, M.G.]]

[[Category: Rossmann, M G.]]

[[Category: Zhang, Y.]]

[[Category: cryo em dengue crd dc-sign]]

[[Category: icosahedral virus]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov 12 20~~:59:~~10 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:34:37 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2b6b.gif|left|200px]]<br />
+[[Image:2b6b.gif|left|200px]]<br /><applet load="2b6b" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="2b6b" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="2b6b" />
 '''Cryo EM structure of Dengue complexed with CRD of DC-SIGN'''<br />
 ==Overview==
-Dengue virus (DENV) is a significant human pathogen that causes millions, of infections and results in about 24,000 deaths each year. Dendritic, cell-specific ICAM3 grabbing nonintegrin (DC-SIGN), abundant in immature, dendritic cells, was previously reported as being an ancillary receptor, interacting with the surface of DENV. The structure of DENV in complex, with the carbohydrate recognition domain (CRD) of DC-SIGN was determined, by cryo-electron microscopy at 25 A resolution. One CRD monomer was found, to bind to two glycosylation sites at Asn67 of two neighboring, glycoproteins in each icosahedral asymmetric unit, leaving the third Asn67, residue vacant. The vacancy at the third Asn67 site is a result of the, nonequivalence of the glycoprotein environments, leaving space for the, primary receptor binding to domain III of E. The use of carbohydrate, moieties for receptor binding sites suggests a mechanism for avoiding, immune surveillance.
+Dengue virus (DENV) is a significant human pathogen that causes millions of infections and results in about 24,000 deaths each year. Dendritic cell-specific ICAM3 grabbing nonintegrin (DC-SIGN), abundant in immature dendritic cells, was previously reported as being an ancillary receptor interacting with the surface of DENV. The structure of DENV in complex with the carbohydrate recognition domain (CRD) of DC-SIGN was determined by cryo-electron microscopy at 25 A resolution. One CRD monomer was found to bind to two glycosylation sites at Asn67 of two neighboring glycoproteins in each icosahedral asymmetric unit, leaving the third Asn67 residue vacant. The vacancy at the third Asn67 site is a result of the nonequivalence of the glycoprotein environments, leaving space for the primary receptor binding to domain III of E. The use of carbohydrate moieties for receptor binding sites suggests a mechanism for avoiding immune surveillance.
 ==About this Structure==
-B6B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Dengue_virus Dengue virus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2B6B OCA].
+B6B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Dengue_virus Dengue virus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B6B OCA].
 ==Reference==
@@ Line 15: / Line 14: @@
 [[Category: Homo sapiens]]
 [[Category: Protein complex]]
-[[Category: Bator-Kelly, C.M.]]
+[[Category: Bator-Kelly, C M.]]
-[[Category: Battisti, A.J.]]
+[[Category: Battisti, A J.]]
-[[Category: Chipman, P.R.]]
+[[Category: Chipman, P R.]]
 [[Category: Gregorio, G.]]
-[[Category: Hendrickson, W.A.]]
+[[Category: Hendrickson, W A.]]
-[[Category: Kuhn, R.J.]]
+[[Category: Kuhn, R J.]]
 [[Category: Pokidysheva, E.]]
-[[Category: Rossmann, M.G.]]
+[[Category: Rossmann, M G.]]
 [[Category: Zhang, Y.]]
 [[Category: cryo em dengue crd dc-sign]]
 [[Category: icosahedral virus]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:59:10 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:34:37 2008''