1qk7: Difference between revisions

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[[Image:1qk7.jpg|left|200px]]<br /><applet load="1qk7" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1qk7.jpg|left|200px]]<br /><applet load="1qk7" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1qk7" />

'''SOLUTION STRUCTURE OF SELENOCOSMIA HUWENA LECTIN-I(SHL-I) BY 2D-NMR'''<br />

==Overview==

The three-dimensional structure of native SHL-I, a lectin from the venom, of the Chinese bird spider Selenocosmia huwena, has been determined from, two-dimensional 1H NMR spectroscopy recorded at 500 and 600 MHz. The best, 10 structures have NOE violation <0.3 A, dihedral violation <2 deg, and, average root-mean-square differences of 0.85 + 0.06 A over backbone atoms., The structure consists of a three-stranded antiparallel beta-sheet and, three turns. The three disulfide bridges and three-stranded antiparallel, beta-sheet form a inhibitor cystine knot motif which is adopted by several, other small proteins, such as huwentoxin-I, omega-conotoxin, and gurmarin., The C-terminal fragment from Leu28 to Trp32 adopts two sets of, conformations corresponding to the cis and trans conformations of Pro31., The structure of SHL-I also has high similarity with that of the, N-terminus of hevein, a lectin from rubber-tree latex.

The three-dimensional structure of native SHL-I, a lectin from the venom of the Chinese bird spider Selenocosmia huwena, has been determined from two-dimensional 1H NMR spectroscopy recorded at 500 and 600 MHz. The best 10 structures have NOE violation <0.3 A, dihedral violation <2 deg, and average root-mean-square differences of 0.85 + 0.06 A over backbone atoms. The structure consists of a three-stranded antiparallel beta-sheet and three turns. The three disulfide bridges and three-stranded antiparallel beta-sheet form a inhibitor cystine knot motif which is adopted by several other small proteins, such as huwentoxin-I, omega-conotoxin, and gurmarin. The C-terminal fragment from Leu28 to Trp32 adopts two sets of conformations corresponding to the cis and trans conformations of Pro31. The structure of SHL-I also has high similarity with that of the N-terminus of hevein, a lectin from rubber-tree latex.

==About this Structure==

1QK7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ornithoctonus_huwena Ornithoctonus huwena]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1QK7 OCA].

1QK7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ornithoctonus_huwena Ornithoctonus huwena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK7 OCA].

==Reference==

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[[Category: shl-i]]

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@@ Line 1: / Line 1: @@
-[[Image:1qk7.jpg|left|200px]]<br /><applet load="1qk7" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1qk7.jpg|left|200px]]<br /><applet load="1qk7" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1qk7" />
 '''SOLUTION STRUCTURE OF SELENOCOSMIA HUWENA LECTIN-I(SHL-I) BY 2D-NMR'''<br />
 ==Overview==
-The three-dimensional structure of native SHL-I, a lectin from the venom, of the Chinese bird spider Selenocosmia huwena, has been determined from, two-dimensional 1H NMR spectroscopy recorded at 500 and 600 MHz. The best, 10 structures have NOE violation &lt;0.3 A, dihedral violation &lt;2 deg, and, average root-mean-square differences of 0.85 + 0.06 A over backbone atoms., The structure consists of a three-stranded antiparallel beta-sheet and, three turns. The three disulfide bridges and three-stranded antiparallel, beta-sheet form a inhibitor cystine knot motif which is adopted by several, other small proteins, such as huwentoxin-I, omega-conotoxin, and gurmarin., The C-terminal fragment from Leu28 to Trp32 adopts two sets of, conformations corresponding to the cis and trans conformations of Pro31., The structure of SHL-I also has high similarity with that of the, N-terminus of hevein, a lectin from rubber-tree latex.
+The three-dimensional structure of native SHL-I, a lectin from the venom of the Chinese bird spider Selenocosmia huwena, has been determined from two-dimensional 1H NMR spectroscopy recorded at 500 and 600 MHz. The best 10 structures have NOE violation &lt;0.3 A, dihedral violation &lt;2 deg, and average root-mean-square differences of 0.85 + 0.06 A over backbone atoms. The structure consists of a three-stranded antiparallel beta-sheet and three turns. The three disulfide bridges and three-stranded antiparallel beta-sheet form a inhibitor cystine knot motif which is adopted by several other small proteins, such as huwentoxin-I, omega-conotoxin, and gurmarin. The C-terminal fragment from Leu28 to Trp32 adopts two sets of conformations corresponding to the cis and trans conformations of Pro31. The structure of SHL-I also has high similarity with that of the N-terminus of hevein, a lectin from rubber-tree latex.
 ==About this Structure==
-QK7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ornithoctonus_huwena Ornithoctonus huwena]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QK7 OCA].
+QK7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ornithoctonus_huwena Ornithoctonus huwena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK7 OCA].
 ==Reference==
@@ Line 21: / Line 21: @@
 [[Category: shl-i]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:46:01 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:40:33 2008''